Shotgun cloning and characterization of the thymidylate synthase- encoding gene from Mycobacterium bovis BCG

Sohkichi Matsumoto; Hideharu Yukitake; Naoya Ohara; Tohru Dairi; Hiroji Kanbara; Takeshi Yamada

doi:10.1111/j.1348-0421.1998.tb01964.x

Shotgun cloning and characterization of the thymidylate synthase- encoding gene from Mycobacterium bovis BCG

Sohkichi Matsumoto, Hideharu Yukitake, Naoya Ohara, Tohru Dairi, Hiroji Kanbara, Takeshi Yamada

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine-depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)-encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.

Original language	English
Pages (from-to)	15-21
Number of pages	7
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	42
Issue number	1
DOIs	https://doi.org/10.1111/j.1348-0421.1998.tb01964.x
Publication status	Published - 1998
Externally published	Yes

Keywords

Gene expression
Mycobacterial enzyme
ThyA

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.1998.tb01964.x

Cite this

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abstract = "The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine-depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)-encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.",

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T1 - Shotgun cloning and characterization of the thymidylate synthase- encoding gene from Mycobacterium bovis BCG

AU - Matsumoto, Sohkichi

AU - Yukitake, Hideharu

AU - Ohara, Naoya

AU - Dairi, Tohru

AU - Kanbara, Hiroji

AU - Yamada, Takeshi

PY - 1998

Y1 - 1998

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AB - The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine-depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)-encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.

KW - Gene expression

KW - Mycobacterial enzyme

KW - ThyA

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Shotgun cloning and characterization of the thymidylate synthase- encoding gene from Mycobacterium bovis BCG

Abstract

Keywords

ASJC Scopus subject areas

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