Simplified culture method of detached ears and its application to vernalization in wheat

K. Kato, S. Tomo, S. Yamazaki, K. Hayashi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The effects of the addition of sulfurous acid into culture solution and of cold treatment of the solution were examined to simplify the culture of detached wheat ears. In the simplified method, detached ears could be cultured at room temperature on the liquid medium containing 100 g/l sucrose and 0.075% sulfurous acid without any sterilization. The immature seeds in detached ears cultured by this method were treated with low temperature or with chemicals known to have vernalizing effect. The chemical treatment did not affect the chilling requirement of immature embryos, although photoperiodic response and narrow-sense earliness were reduced by kinetin and trypsin. The low temperature treatment drastically affected the chilling requirement, and fully vernalized mature seeds having normal germinability were obtained by treating the detached ears in culture with low temperature from 10 days after anthesis.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
Issue number2
Publication statusPublished - Aug 1990
Externally publishedYes


  • Triticum aestivum
  • bread wheat
  • detached ear culture
  • immature seed vernalization
  • narrow-sense earliness
  • photoperiodic response

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture


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