TY - GEN
T1 - Sol-gel preparation of HAp-coated silica macrospheres from water glass and their protein adsorption
AU - Li, Jie
AU - Shirosaki, Yuki
AU - Hayakawa, Satoshi
AU - Stamboulis, Artemis
AU - Osaka, Akiyoshi
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - Silica gel macrospheres of 2~4 mm in size, and wrapped with Ca-alginate chelate film were prepared as the substrate for hydroxyapatite coating layer, using water glass as the silica source. Those Ca-SiO2 macrospheres were soaked in a 1:1 (volume) mixture of ethanol and 0.1 M Na 2HPO4 to deposit hydroxyapatite layer (HAp-SiO2 macrospheres). Adsorption of bovine serum albumin and egg lysozyme on those Ca-SiO2 and HAp-SiO2 macrospheres under physiological pH (7.2) was well correlated to the Langmuir-type adsorption equation. The electrostatic interactions between the protein molecules and those macrospheres well interpret the adsorption isotherms, while the mesopores in the Ca-SiO 2contributed to some extent. A multi-layer adsorption model was proposed.
AB - Silica gel macrospheres of 2~4 mm in size, and wrapped with Ca-alginate chelate film were prepared as the substrate for hydroxyapatite coating layer, using water glass as the silica source. Those Ca-SiO2 macrospheres were soaked in a 1:1 (volume) mixture of ethanol and 0.1 M Na 2HPO4 to deposit hydroxyapatite layer (HAp-SiO2 macrospheres). Adsorption of bovine serum albumin and egg lysozyme on those Ca-SiO2 and HAp-SiO2 macrospheres under physiological pH (7.2) was well correlated to the Langmuir-type adsorption equation. The electrostatic interactions between the protein molecules and those macrospheres well interpret the adsorption isotherms, while the mesopores in the Ca-SiO 2contributed to some extent. A multi-layer adsorption model was proposed.
KW - Hydroxyapatite coating
KW - Protein adsorption
KW - Silica gel macrosphere
KW - Water glass
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U2 - 10.4028/www.scientific.net/KEM.529-530.637
DO - 10.4028/www.scientific.net/KEM.529-530.637
M3 - Conference contribution
AN - SCOPUS:84871314171
SN - 9783037855171
T3 - Key Engineering Materials
SP - 637
EP - 640
BT - Bioceramics 24
PB - Trans Tech Publications Ltd
T2 - 24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
Y2 - 21 October 2012 through 24 October 2012
ER -