TY - JOUR
T1 - Specific antiviral effect of violaceoid E on bovine leukemia virus
AU - Murakami, Hironobu
AU - Murakami-Kawai, Makoto
AU - Kamisuki, Shinji
AU - Hisanobu, Shibasaki
AU - Tsurukawa, Yukine
AU - Uchiyama, Jumpei
AU - Sakaguchi, Masahiro
AU - Tsukamoto, Kenji
N1 - Funding Information:
This study was supported by a research project grant awarded by the Ito Foundation (Tokyo, Japan), the Azabu University Research Services Division , Ministry of Education, Culture, Sports, Science and Technology (MEXT) Supported Program for the Private University Research Branding Project , 2016–2019, Center for Human and Animal Symbiosis Science , Azabu University, and JSPS KAKENHI [grant number 19K06410 ].
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/10
Y1 - 2021/10
N2 - Bovine leukemia virus (BLV) infection has spread worldwide causing significant economic losses in the livestock industry. In countries with a high prevalence of BLV, minimizing economic losses is challenging; thus, research into various countermeasures is important for improving BLV control. Because anti-BLV drugs have not been developed, the present study explored a promising chemical compound with anti-BLV activity. Initially, screening of a chemical compound library revealed that violaceoid E (vioE), which is isolated from fungus, showed antiviral activity. Further analysis demonstrated that the antiviral effect of vioE inhibited transcriptional activation of BLV. Cellular thermal shift assay and pulldown assays provided evidence for a direct interaction between vioE and the viral transactivator protein, Tax. These data indicate that interference with Tax-dependent transcription could be a novel target for development of anti-BLV drugs. Therefore, it is suggested that vioE is a novel antiviral compound against BLV.
AB - Bovine leukemia virus (BLV) infection has spread worldwide causing significant economic losses in the livestock industry. In countries with a high prevalence of BLV, minimizing economic losses is challenging; thus, research into various countermeasures is important for improving BLV control. Because anti-BLV drugs have not been developed, the present study explored a promising chemical compound with anti-BLV activity. Initially, screening of a chemical compound library revealed that violaceoid E (vioE), which is isolated from fungus, showed antiviral activity. Further analysis demonstrated that the antiviral effect of vioE inhibited transcriptional activation of BLV. Cellular thermal shift assay and pulldown assays provided evidence for a direct interaction between vioE and the viral transactivator protein, Tax. These data indicate that interference with Tax-dependent transcription could be a novel target for development of anti-BLV drugs. Therefore, it is suggested that vioE is a novel antiviral compound against BLV.
KW - Antiviral activity
KW - Antiviral drugs
KW - Bovine leukemia virus
KW - Chemical compound
KW - Tax protein
KW - Transcription
KW - Violaceoid E
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U2 - 10.1016/j.virol.2021.06.010
DO - 10.1016/j.virol.2021.06.010
M3 - Article
C2 - 34242747
AN - SCOPUS:85109159470
SN - 0042-6822
VL - 562
SP - 1
EP - 8
JO - Virology
JF - Virology
ER -