Spectrophotometric Determination of copper(ii) with o-Bromophenylfluorone

Takako Yamaguchi, Megumi Samma, Shinichiro Kamino, Momoka Matsushita, Tomoyuki Hashimoto, Yoshikazu Fujita

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A simple, reliable and sensitive spectrophotometric method for the determination of copper(II) was established with o-bromophenylfluorone (OBPF), a novel chemical probe, in the presence of poly(N-vinylpyrrolidone). Beer's law was obeyed in the range of 8 - 160 ng ml-1, with an apparent molar absorptivity at 570 nm, the relative standard deviation being 3.64 × 105 dm3 mol-1 cm-1 and 0.72% (n = 5). This method was applied to the recovery tests of copper(II) in human urine, bovine serum and tap water; the results were satisfactory. 2009

Original languageEnglish
Pages (from-to)1457-1460
Number of pages4
JournalAnalytical Sciences
Issue number12
Publication statusPublished - Dec 2009
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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