Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex

Masahito Naito; Hiroto Nakashima; Masanobu Ohki; Shinichirou Kamino; Takako Yamaguchi; Yoshikazu Fujita

doi:10.2116/bunsekikagaku.56.781

Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex

Masahito Naito, Hiroto Nakashima, Masanobu Ohki, Shinichirou Kamino, Takako Yamaguchi, Yoshikazu Fujita

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL ^-1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 ⁴ dm ³ mol ^-1 cm ^-1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.

Original language	English
Pages (from-to)	781-784
Number of pages	4
Journal	BUNSEKI KAGAKU
Volume	56
Issue number	9
DOIs	https://doi.org/10.2116/bunsekikagaku.56.781
Publication status	Published - 2007

Keywords

Insuline
Metabolic syndrome
Spectrophotometry
Ternary complex formation
o-carboxyphenylfluorone

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/bunsekikagaku.56.781

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title = "Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex",

abstract = "The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.",

keywords = "Insuline, Metabolic syndrome, Spectrophotometry, Ternary complex formation, o-carboxyphenylfluorone",

author = "Masahito Naito and Hiroto Nakashima and Masanobu Ohki and Shinichirou Kamino and Takako Yamaguchi and Yoshikazu Fujita",

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T1 - Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex

AU - Naito, Masahito

AU - Nakashima, Hiroto

AU - Ohki, Masanobu

AU - Kamino, Shinichirou

AU - Yamaguchi, Takako

AU - Fujita, Yoshikazu

PY - 2007

Y1 - 2007

N2 - The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.

AB - The method is based on ternary complex formation among o- carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL -1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 10 4 dm 3 mol -1 cm -1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.

KW - Insuline

KW - Metabolic syndrome

KW - Spectrophotometry

KW - Ternary complex formation

KW - o-carboxyphenylfluorone

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JO - Bunseki Kagaku

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Spectrophotometric determination of insuline by ternary complex formation with o-carboxyphenylfluorone-copper(II) complex

Abstract

Keywords

ASJC Scopus subject areas

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