Spectrophotometric Determination of Phosphate Ion with Ferric Salt of Tetraphenylporphine-trisulfonic Acid

Yutaka Saito; Masaki Mifune; Kenji Ikegami; Kimiya Okazaki; Junichi Odo; Yoshimasa Tanaka

doi:10.1248/cpb.33.3381

Spectrophotometric Determination of Phosphate Ion with Ferric Salt of Tetraphenylporphine-trisulfonic Acid

Yutaka Saito, Masaki Mifune, Kenji Ikegami, Kimiya Okazaki, Junichi Odo, Yoshimasa Tanaka

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A new iadirect spectrophotometric method for the determination of phosphate ion by the use of solid ferric salt of tetraphenylporphine-trisulfonic acid (TPPSFe) was developed. The method is based on the measurement of the absorbance of TPPS liberated from TPPSFe at its Q band (procedure A) or its Soret band (procedure B). Phosphate ion ranging from 10 to 50 pg and from 4 to 20μg could be determined based on the absorption coefficients of 8.2 x 10³ at 512 nm (procedure A) and 2.2 x 10⁵ at 434 nm (procedure B). The advantages of this method are its simple procedure and mild conditions. Procedure A was successfully applied to the determination of phosphate ion liberated from glucose-1-phosphate by acid phosphatase.

Original language	English
Pages (from-to)	3381-3384
Number of pages	4
Journal	Chemical and Pharmaceutical Bulletin
Volume	33
Issue number	8
DOIs	https://doi.org/10.1248/cpb.33.3381
Publication status	Published - 1985

Keywords

acid phosphatase
determination of phosphate ion
ferric salt glucose-1 -phosphate
indirect spectrophotometry
tetraphenylporphine-trisulfonic acid

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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title = "Spectrophotometric Determination of Phosphate Ion with Ferric Salt of Tetraphenylporphine-trisulfonic Acid",

abstract = "A new iadirect spectrophotometric method for the determination of phosphate ion by the use of solid ferric salt of tetraphenylporphine-trisulfonic acid (TPPSFe) was developed. The method is based on the measurement of the absorbance of TPPS liberated from TPPSFe at its Q band (procedure A) or its Soret band (procedure B). Phosphate ion ranging from 10 to 50 pg and from 4 to 20μg could be determined based on the absorption coefficients of 8.2 x 103 at 512 nm (procedure A) and 2.2 x 105 at 434 nm (procedure B). The advantages of this method are its simple procedure and mild conditions. Procedure A was successfully applied to the determination of phosphate ion liberated from glucose-1-phosphate by acid phosphatase.",

keywords = "acid phosphatase, determination of phosphate ion, ferric salt glucose-1 -phosphate, indirect spectrophotometry, tetraphenylporphine-trisulfonic acid",

author = "Yutaka Saito and Masaki Mifune and Kenji Ikegami and Kimiya Okazaki and Junichi Odo and Yoshimasa Tanaka",

year = "1985",

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T1 - Spectrophotometric Determination of Phosphate Ion with Ferric Salt of Tetraphenylporphine-trisulfonic Acid

AU - Saito, Yutaka

AU - Mifune, Masaki

AU - Ikegami, Kenji

AU - Okazaki, Kimiya

AU - Odo, Junichi

AU - Tanaka, Yoshimasa

PY - 1985

Y1 - 1985

N2 - A new iadirect spectrophotometric method for the determination of phosphate ion by the use of solid ferric salt of tetraphenylporphine-trisulfonic acid (TPPSFe) was developed. The method is based on the measurement of the absorbance of TPPS liberated from TPPSFe at its Q band (procedure A) or its Soret band (procedure B). Phosphate ion ranging from 10 to 50 pg and from 4 to 20μg could be determined based on the absorption coefficients of 8.2 x 103 at 512 nm (procedure A) and 2.2 x 105 at 434 nm (procedure B). The advantages of this method are its simple procedure and mild conditions. Procedure A was successfully applied to the determination of phosphate ion liberated from glucose-1-phosphate by acid phosphatase.

AB - A new iadirect spectrophotometric method for the determination of phosphate ion by the use of solid ferric salt of tetraphenylporphine-trisulfonic acid (TPPSFe) was developed. The method is based on the measurement of the absorbance of TPPS liberated from TPPSFe at its Q band (procedure A) or its Soret band (procedure B). Phosphate ion ranging from 10 to 50 pg and from 4 to 20μg could be determined based on the absorption coefficients of 8.2 x 103 at 512 nm (procedure A) and 2.2 x 105 at 434 nm (procedure B). The advantages of this method are its simple procedure and mild conditions. Procedure A was successfully applied to the determination of phosphate ion liberated from glucose-1-phosphate by acid phosphatase.

KW - acid phosphatase

KW - determination of phosphate ion

KW - ferric salt glucose-1 -phosphate

KW - indirect spectrophotometry

KW - tetraphenylporphine-trisulfonic acid

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Spectrophotometric Determination of Phosphate Ion with Ferric Salt of Tetraphenylporphine-trisulfonic Acid

Abstract

Keywords

ASJC Scopus subject areas

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