Spectrophotometric determination of uric acid based on fading of o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex

Takako Yamaguchi; Kohei Hasegawa; Shinichiro Kamino; Kanako Miyachi; Hiroshi Tominaga; Yoshikazu Fujita

doi:10.2116/analsci.23.223

Spectrophotometric determination of uric acid based on fading of o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex

Takako Yamaguchi, Kohei Hasegawa, Shinichiro Kamino, Kanako Miyachi, Hiroshi Tominaga, Yoshikazu Fujita

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01 - 0.20 μg ml^-1, with an effective molar absorptivity at 635 nm, the relative standard deviation being 6.5 × 10⁵ dm³ mol^-1 cm^-1 and 1.5% (n = 5). This method is about 20-times more sensitive than the conventional methods. The method was successfully applied to the assay of UA in human urine. 2007

Original language	English
Pages (from-to)	223-226
Number of pages	4
Journal	Analytical Sciences
Volume	23
Issue number	2
DOIs	https://doi.org/10.2116/analsci.23.223
Publication status	Published - Feb 2007
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01 - 0.20 μg ml-1, with an effective molar absorptivity at 635 nm, the relative standard deviation being 6.5 × 105 dm3 mol-1 cm-1 and 1.5% (n = 5). This method is about 20-times more sensitive than the conventional methods. The method was successfully applied to the assay of UA in human urine. 2007",

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AU - Yamaguchi, Takako

AU - Hasegawa, Kohei

AU - Kamino, Shinichiro

AU - Miyachi, Kanako

AU - Tominaga, Hiroshi

AU - Fujita, Yoshikazu

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Spectrophotometric determination of uric acid based on fading of o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex

Abstract

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