Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation

Yuki Morimoto; Mamoru Ouchida; Toshifumi Ozaki; Akira Kawai; Tatsuo Ito; Aki Yoshida; Hajime Inoue; Kenji Shimizu

doi:10.1016/S0304-3835(03)00314-8

Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation

Yuki Morimoto, Mamoru Ouchida, Toshifumi Ozaki, Akira Kawai, Tatsuo Ito, Aki Yoshida, Hajime Inoue, Kenji Shimizu

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.

Original language	English
Pages (from-to)	35-43
Number of pages	9
Journal	Cancer Letters
Volume	199
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(03)00314-8
Publication status	Published - Sept 10 2003

Keywords

Alternative splicing
Cell growth
SSX
SYT
Synovial sarcoma
Transcriptional activity

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(03)00314-8

Cite this

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title = "Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation",

abstract = "The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.",

keywords = "Alternative splicing, Cell growth, SSX, SYT, Synovial sarcoma, Transcriptional activity",

author = "Yuki Morimoto and Mamoru Ouchida and Toshifumi Ozaki and Akira Kawai and Tatsuo Ito and Aki Yoshida and Hajime Inoue and Kenji Shimizu",

note = "Funding Information: This work was supported by a Grant-in-Aid for Cancer Research from the Ministry of Health, Labor and Welfare to A.K., and by Grant-in-Aid from the Ministry of Education, Science, Sports and Culture and from the Ministry of Health and Welfare of Japan to K.S.",

year = "2003",

month = sep,

day = "10",

doi = "10.1016/S0304-3835(03)00314-8",

language = "English",

volume = "199",

pages = "35--43",

journal = "Cancer Letters",

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TY - JOUR

T1 - Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation

AU - Morimoto, Yuki

AU - Ouchida, Mamoru

AU - Ozaki, Toshifumi

AU - Kawai, Akira

AU - Ito, Tatsuo

AU - Yoshida, Aki

AU - Inoue, Hajime

AU - Shimizu, Kenji

N1 - Funding Information: This work was supported by a Grant-in-Aid for Cancer Research from the Ministry of Health, Labor and Welfare to A.K., and by Grant-in-Aid from the Ministry of Education, Science, Sports and Culture and from the Ministry of Health and Welfare of Japan to K.S.

PY - 2003/9/10

Y1 - 2003/9/10

N2 - The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.

AB - The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.

KW - Alternative splicing

KW - Cell growth

KW - SSX

KW - SYT

KW - Synovial sarcoma

KW - Transcriptional activity

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UR - http://www.scopus.com/inward/citedby.url?scp=0042881136&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(03)00314-8

DO - 10.1016/S0304-3835(03)00314-8

M3 - Article

C2 - 12963121

AN - SCOPUS:0042881136

SN - 0304-3835

VL - 199

SP - 35

EP - 43

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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