Spontaneous Structural Changes in Actin Regulate G-F Transformation

Masatoshi Morimatsu; Yuichi Togashi; So Nishikawa; Mitsuhiro Sugawa; Atsuko H. Iwane; Toshio Yanagida

doi:10.1371/journal.pone.0045864

Spontaneous Structural Changes in Actin Regulate G-F Transformation

Masatoshi Morimatsu, Yuichi Togashi, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Transformations between G- (monomeric) and F-actin (polymeric) are important in cellular behaviors such as migration, cytokinesis, and morphing. In order to understand these transitions, we combined single-molecule Förster resonance energy transfer with total internal reflection fluorescence microscopy to examine conformational changes of individual actin protomers. We found that the protomers can take different conformational states and that the transition interval is in the range of hundreds of seconds. The distribution of these states was dependent on the environment, suggesting that actin undergoes spontaneous structural changes that accommodate itself to polymerization.

Original language	English
Article number	e45864
Journal	PloS one
Volume	7
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0045864
Publication status	Published - Nov 5 2012
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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AU - Nishikawa, So

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AU - Iwane, Atsuko H.

AU - Yanagida, Toshio

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AB - Transformations between G- (monomeric) and F-actin (polymeric) are important in cellular behaviors such as migration, cytokinesis, and morphing. In order to understand these transitions, we combined single-molecule Förster resonance energy transfer with total internal reflection fluorescence microscopy to examine conformational changes of individual actin protomers. We found that the protomers can take different conformational states and that the transition interval is in the range of hundreds of seconds. The distribution of these states was dependent on the environment, suggesting that actin undergoes spontaneous structural changes that accommodate itself to polymerization.

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Spontaneous Structural Changes in Actin Regulate G-F Transformation

Abstract

ASJC Scopus subject areas

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