TY - JOUR
T1 - Stable expression of the neuronal BI (class A) calcium channel in baby hamster kidney cells
AU - Niidome, Tetsuhiro
AU - Teramoto, Tetsuyuki
AU - Murata, Yoshiyuki
AU - Tanaka, Isao
AU - Seto, Toshio
AU - Sawada, Kohei
AU - Mori, Yasuo
AU - Katayama, Kouichi
PY - 1994/9/30
Y1 - 1994/9/30
N2 - Plasmids containing the BI (α1) cDNA with the dihydrofolate reductase (DHFR) gene, skeletal muscle α2-subunit cDNA with the neo marker gene, and β-subunit cDNA were co-transfected into baby hamster kidney (BHK) cells. BHK cells lack endogenous calcium channel activity. Twenty percent of the methotrexate (MTX) and G418 resistant clones were found to express calcium channel activity using the patch-clamp technique. A single clone, BHKBI147, demonstrated stable electrophysiological characteristics over 20 passages. Ca2+ currents of the BI channel in BHKBI147 cells were largely blocked by a specific P-type blocker, ω-AgaIVA, with an IC50 of 150 nM. Unlike the BI channel, Ca2+ currents of cardiac L-type channels expressed in BHK cells were completely blocked by the L-type antagonist, nifedipine, with an IC50 of 56 nM.
AB - Plasmids containing the BI (α1) cDNA with the dihydrofolate reductase (DHFR) gene, skeletal muscle α2-subunit cDNA with the neo marker gene, and β-subunit cDNA were co-transfected into baby hamster kidney (BHK) cells. BHK cells lack endogenous calcium channel activity. Twenty percent of the methotrexate (MTX) and G418 resistant clones were found to express calcium channel activity using the patch-clamp technique. A single clone, BHKBI147, demonstrated stable electrophysiological characteristics over 20 passages. Ca2+ currents of the BI channel in BHKBI147 cells were largely blocked by a specific P-type blocker, ω-AgaIVA, with an IC50 of 150 nM. Unlike the BI channel, Ca2+ currents of cardiac L-type channels expressed in BHK cells were completely blocked by the L-type antagonist, nifedipine, with an IC50 of 56 nM.
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U2 - 10.1006/bbrc.1994.2399
DO - 10.1006/bbrc.1994.2399
M3 - Article
AN - SCOPUS:0027967520
SN - 0006-291X
VL - 203
SP - 1821
EP - 1827
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -