Staining air dried protoplasts for study of plant chromosomes

M. Murata

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)


    The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poorly stained by common stains, and difficult to spread by the squash technique. In this study, however, the chromosomes of B. carinata in callus culture were spread well and stained clearly with Giemsa staining solution. The chromosome preparations by the present techniques should also be amenable to chromosome banding studies in plants.

    Original languageEnglish
    Pages (from-to)101-106
    Number of pages6
    JournalBiotechnic and Histochemistry
    Issue number2
    Publication statusPublished - Jan 1 1983

    ASJC Scopus subject areas

    • Histology
    • Medical Laboratory Technology


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