Standardization of Switchgrass Sample Collection for Cell Wall and Biomass Trait Analysis

C. Frank Hardin, Chunxiang Fu, Hiroshi Hisano, Xirong Xiao, Hui Shen, C. Neal Stewart, Wayne Parrott, Richard A. Dixon, Zeng Yu Wang

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)


As a native, low-input crop with high biomass production, switchgrass (Panicum virgatum) has become a favorable feedstock for the production of cellulosic biofuels in the United States. Many efforts are being made to improve the production of cellulosic biofuels from switchgrass. Protocols regarding analysis of switchgrass biomass have been established; however, the developmental stage of the materials being analyzed has varied depending on researchers' discretion, and no standardized harvesting procedure has been defined. Developmental stages have a large impact on the results of biochemical analyses. We propose a standardized procedure for switchgrass sample collection for cell wall and biomass analyses by describing various developmental stages of switchgrass, defining the R1 stage as the stage at which tillers should be collected, and providing a detailed description of how and what material should be analyzed. Such a standardized procedure will help to maintain consistency in switchgrass evaluation methods, enable comparisons of data obtained from different approaches and studies, and facilitate efforts towards improving switchgrass as a bioenergy crop.

Original languageEnglish
Pages (from-to)755-762
Number of pages8
JournalBioenergy Research
Issue number2
Publication statusPublished - Jun 2013
Externally publishedYes


  • Cellulosic biofuels
  • Developmental stage
  • Panicum virgatum
  • Sample collection
  • Switchgrass

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Energy (miscellaneous)


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