Strain-dependent induction of allergic rhinitis without adjuvant in mice

D. A. Harn, M. Abe, M. M. Wang, A. R. Satoskar, Kazunori Nishizaki, Y. Masuda, T. Yoshino, Mitsuhiro Okano

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)


Background: To date, no murine models have been reported to show the induction of both antigen-specific IgE and nasal eosinophilia, two of the major hallmarks of allergic rhinitis, after local sensitization in the absence of adjuvants, a phenomenon which reflects natural exposure. In this report, we attempted to establish a murine model representing an initiation of allergic rhinitis. Methods: BALB/c, CBA/J, and C57BL/6 mice were sensitized intranasally to Schistosoma mansoni egg antigen (SEA) solely. After repeated sensitization, serum Ab titers, nasal eosinophilia, and cytokine production by nasal lymphocytes were determined. Results: BALB/c mice produced SEA-specific IgE after repeated sensitization. High-dose sensitization to SEA induced IgE production in CBA/J mice, while C57BL/6 mice did not show the production throughout the period observed, suggesting that IgE production was regulated genetically. BALB/c mice also exhibited nasal eosinophilia after the nasal challenge. In addition, nasal lymphocytes sensitized with SEA intranasally produced significant amount of IL-5 in vitro. Conclusions: These results suggest that intranasal sensitization with SEA in the absence of adjuvants induces a Th2 immune reaction, reflecting the hallmarks of the initiation of allergic rhinitis both in vivo and in vitro, which is genetically regulated.

Original languageEnglish
Pages (from-to)593-601
Number of pages9
JournalAllergy: European Journal of Allergy and Clinical Immunology
Issue number6
Publication statusPublished - Jul 10 1999


  • Adjuvant
  • Allergic rhinitis
  • Cytokine
  • Eosinophilia
  • IgE
  • Intranasal sensitization
  • Mouse
  • Strain

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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