Stretching of single DNA molecules caused by accelerating flow on a microchip

Ken Hirano, Takafumi Iwaki, Tomomi Ishido, Yuko Yoshikawa, Keiji Naruse, Kenichi Yoshikawa

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


DNA elongation induced by fluidic stress was investigated on a microfluidic chip composed of a large inlet pool and a narrow channel. Through single-DNA observation with fluorescence microscopy, the manner of stretching of individual T4 DNA molecules (166 kbp) was monitored near the area of accelerating flow with narrowing streamlines. The results showed that the DNA long-axis length increased in a sigmoidal manner depending on the magnitude of flow acceleration, or shear, along the DNA chain. To elucidate the physical mechanism of DNA elongation, we performed a theoretical study by adopting a model of a coarse-grained nonlinear elastic polymer chain elongated by shear stress due to acceleration flow along the chain direction.

Original languageEnglish
Article number165101
JournalJournal of Chemical Physics
Issue number16
Publication statusPublished - Oct 28 2018

ASJC Scopus subject areas

  • General Physics and Astronomy
  • Physical and Theoretical Chemistry


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