TY - JOUR
T1 - Structural basis for high selectivity of a rice silicon channel Lsi1
AU - Saitoh, Yasunori
AU - Mitani-Ueno, Namiki
AU - Saito, Keisuke
AU - Matsuki, Kengo
AU - Huang, Sheng
AU - Yang, Lingli
AU - Yamaji, Naoki
AU - Ishikita, Hiroshi
AU - Shen, Jian Ren
AU - Ma, Jian Feng
AU - Suga, Michihiro
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grants JP16H06296 (M.S. and J.F.M.), JP21H05034 (M.S. and J.F.M.), JP17H06879 (Y.S.), JP19K16056 (Y.S.), 18H05155 (H.I.), 18H01937 (H.I.), 20H03217 (H.I.), 20H05090 (H.I.), 16H06560 (K.S.) and 18H01186 (K.S.). This work was also supported by JST CREST JPMJCR1656 (H.I.) and the Interdisciplinary Computational Science Program in CCS, University of Tsukuba. The X-ray diffraction experiment was performed at beamlines 41XU and 44XU of SPring-8 (Hyogo, Japan) with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (proposals 2016B6621, 2017A6724, 2017B6724, 2018A2530, 2018B2530, 2019A2559 and 2019B2559), and we thank the staff at SPring-8 for their help. We thank S. Yonekura for the discussion, A. Morita, Y. Takahashi, M. Hikasa and S. Rikiishi for experimental assistance.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Silicon (Si), the most abundant mineral element in the earth’s crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.
AB - Silicon (Si), the most abundant mineral element in the earth’s crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.
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U2 - 10.1038/s41467-021-26535-x
DO - 10.1038/s41467-021-26535-x
M3 - Article
C2 - 34716344
AN - SCOPUS:85118464863
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 6236
ER -