TY - JOUR
T1 - Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin
AU - Konishi, Tomoyuki
AU - Taguchi, Fumiko
AU - Iwaki, Masako
AU - Ohnishi-Kameyama, Mayumi
AU - Yamamoto, Masanobu
AU - Maeda, Ikuko
AU - Nishida, Yoshihiro
AU - Ichinose, Yuki
AU - Yoshida, Mitsuru
AU - Ishii, Tadashi
PY - 2009/11/2
Y1 - 2009/11/2
N2 - The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-l-Rha-(1→, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rha-(1→, which was identified in a previous study.
AB - The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-l-Rha-(1→, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rha-(1→, which was identified in a previous study.
KW - Bacteria
KW - Flagellin glycosylation
KW - NMR
KW - O-linked oligosaccharides
KW - Pseudomonas syringae
KW - Structure
UR - http://www.scopus.com/inward/record.url?scp=70349774389&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349774389&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2009.07.004
DO - 10.1016/j.carres.2009.07.004
M3 - Article
C2 - 19766989
AN - SCOPUS:70349774389
SN - 0008-6215
VL - 344
SP - 2250
EP - 2254
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 16
ER -