TY - JOUR
T1 - Structural characterization of neutral and anionic glucans from Mesorhizobium loti
AU - Kawaharada, Yasuyuki
AU - Kiyota, Hiromasa
AU - Eda, Shima
AU - Minamisawa, Kiwamu
AU - Mitsui, Hisayuki
N1 - Funding Information:
We thank Mrs. Teiko Yamada (Tohoku University) for technical assistance on NMR spectroscopy. This work was supported in part by Grant-in-Aid for Scientific Research (No. 19580077) from Japan Society for the Promotion of Science, and by Grant-in-Aid for Scientific Research on Priority Areas ‘Comparative Genomics’ from the Ministry of Education, Culture, Sports, Science and Technology.
PY - 2008/9/22
Y1 - 2008/9/22
N2 - The periplasmic glucans of Mesorhizobium loti were isolated and separated into fractions according to their acidity. NMR spectroscopy confirmed their backbone structure to be a cyclic β-(1→2)-d-glucan as in the case of other rhizobia, and revealed no non-glycosidic substituents in the neutral fraction, and glycerophosphoryl and succinyl residues as major and minor substituents, respectively, in the anionic fractions. MALDI-TOF mass spectrometry showed that the anionic glucans contain one, two, or three such substituents per molecule according to their acidity, and, in contrast, that all the anionic subfractions have a similar size distribution to that of the neutral glucans, where molecules composed of 20-24 glucosyl residues are predominant. These results clarify the periplasmic glucan composition in terms of charge-to-mass ratios in M. loti cells.
AB - The periplasmic glucans of Mesorhizobium loti were isolated and separated into fractions according to their acidity. NMR spectroscopy confirmed their backbone structure to be a cyclic β-(1→2)-d-glucan as in the case of other rhizobia, and revealed no non-glycosidic substituents in the neutral fraction, and glycerophosphoryl and succinyl residues as major and minor substituents, respectively, in the anionic fractions. MALDI-TOF mass spectrometry showed that the anionic glucans contain one, two, or three such substituents per molecule according to their acidity, and, in contrast, that all the anionic subfractions have a similar size distribution to that of the neutral glucans, where molecules composed of 20-24 glucosyl residues are predominant. These results clarify the periplasmic glucan composition in terms of charge-to-mass ratios in M. loti cells.
KW - Cyclic glucan
KW - Glycerophosphoryl substituent
KW - Mesorhizobium loti
KW - Succinyl substituent
KW - β-(1→2)-Glycosidic linkage
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U2 - 10.1016/j.carres.2008.07.007
DO - 10.1016/j.carres.2008.07.007
M3 - Article
C2 - 18667198
AN - SCOPUS:49649127584
SN - 0008-6215
VL - 343
SP - 2422
EP - 2427
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 14
ER -