Structure-function relationships in the Mn4CaO5 water-splitting cluster

Jian Ren Shen

doi:10.1007/978-1-4939-1148-6_11

Structure-function relationships in the Mn₄CaO₅ water-splitting cluster

Jian Ren Shen

Research Institute for Interdisciplinary Science

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Mn₄CaO₅ cluster is the catalytic center for photosynthetic water-splitting harbored in photosystem II (PSII), a huge, multi-subunit membrane- protein complex located in the thylakoid membranes from cyanobacteria to higher plants. The structure of PSII has been analyzed at 1.9 Å resolution by X-ray crystallography, revealing a clear picture of the Mn4CaO5 cluster. In this chapter, principles of crystallization and crystal structure analysis are briefly introduced, followed by descriptions of the structure of the Mn₄CaO₅ cluster and its implications in the mechanism of water-splitting. Based on the geometric organization of the Mn₄CaO₅ cluster, the location of four terminal water ligands, the possible oxidation states of the four Mn ions reported so far, as well as the structural changes revealed by replacing the Ca ion with Sr, a mechanism for water-splitting was proposed and discussed.

Original language	English
Title of host publication	The Biophysics of Photosynthesis
Publisher	Springer New York
Pages	321-349
Number of pages	29
ISBN (Electronic)	9781493911486
ISBN (Print)	9781493911479
DOIs	https://doi.org/10.1007/978-1-4939-1148-6_11
Publication status	Published - Jan 1 2014

Keywords

Crystal structure
Crystallization
Membrane proteins
Oxygen evolution
Photosynthesis
Photosystem II
S-state

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-1148-6_11

Cite this

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abstract = "Mn4CaO5 cluster is the catalytic center for photosynthetic water-splitting harbored in photosystem II (PSII), a huge, multi-subunit membrane- protein complex located in the thylakoid membranes from cyanobacteria to higher plants. The structure of PSII has been analyzed at 1.9 {\AA} resolution by X-ray crystallography, revealing a clear picture of the Mn4CaO5 cluster. In this chapter, principles of crystallization and crystal structure analysis are briefly introduced, followed by descriptions of the structure of the Mn4CaO5 cluster and its implications in the mechanism of water-splitting. Based on the geometric organization of the Mn4CaO5 cluster, the location of four terminal water ligands, the possible oxidation states of the four Mn ions reported so far, as well as the structural changes revealed by replacing the Ca ion with Sr, a mechanism for water-splitting was proposed and discussed.",

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