Structures of N-Linked Oligosaccharides of Microsomal Glycoproteins from Developing Castor Bean Endosperms

Yoshinobu Kimura, Toshihiko Tokuda, Masafumi Yamai, Shuhei Nakajima, Eiji Higashide, Shigeaki Takagi

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10 Citations (Scopus)


The structures of sugar chains of the glycoproteins from the microsomal fraction of developing castor bean endosperms have been analyzed. The structural analyses were done by a fluorescence method combined with component analysis, exoglycosidase digestions, partial acetolysis, Smith degradation, and 1H-NMR spectroscopy. The estimated structures fell into three categories; the first was oligomannose-type, the second xylomannose-type, the third complex-type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2 (M3FX) and Man6GlcNAc2 (M6B) were the major structures. The structures of Man4GlcNAc2 (M4C) and Man4Xyl1GlcNAc2 (M4X) have also been found in the microsomal glycoproteins of the developing bean endosperms. These results could indicate that the structures of M4C, M4X, and M3FX are formed in the stage of sugar chain processing in the microsomal fraction, in which oligomannose-type sugar chains are modified into complex-type ones by several kinds of processing enzymes.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalBioscience, biotechnology, and biochemistry
Issue number2
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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