TY - JOUR
T1 - Structures of sugar chains of Ricinus communis agglutinin
AU - Kimura, Yoshinobu
AU - Hase, Sumihiro
AU - Kobayashi, Yuji
AU - Kyogoku, Yoshimasa
AU - Ikenaka, Tokuji
AU - Funatsu, Gunki
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid (No. 61106004) for Special Project Research from the Ministry of Education, Science and Culture of Japan.
PY - 1988/8/11
Y1 - 1988/8/11
N2 - The structures of sugar chains from Ricinus communis agglutinin were determined. Four glycopeptides were isolated from the lectin according to a published method (Kimura, Y. and Funatsu, G. (1988) Agric. Biol. Chem. 52, in press), and sugar chains of each glycopeptide were liberated by hydrazinolysis. Free amino groups were N-acetylated and the reducing-end residues were coupled with 2-aminopyridine. The resulting pyridylamino derivatives of sugar chains were purified by gel filtration and reversed-phase HPLC. The structures of thus-purified PA-sugar chains were determined by a combination of component analysis, stepwise exoglycosidase digestions, partial acetolysis, and 500 MHz 1H-NMR spectroscopy. These results indicate that R. communis agglutinin contains the sugar chains shown on page 249.
AB - The structures of sugar chains from Ricinus communis agglutinin were determined. Four glycopeptides were isolated from the lectin according to a published method (Kimura, Y. and Funatsu, G. (1988) Agric. Biol. Chem. 52, in press), and sugar chains of each glycopeptide were liberated by hydrazinolysis. Free amino groups were N-acetylated and the reducing-end residues were coupled with 2-aminopyridine. The resulting pyridylamino derivatives of sugar chains were purified by gel filtration and reversed-phase HPLC. The structures of thus-purified PA-sugar chains were determined by a combination of component analysis, stepwise exoglycosidase digestions, partial acetolysis, and 500 MHz 1H-NMR spectroscopy. These results indicate that R. communis agglutinin contains the sugar chains shown on page 249.
KW - Glycoprotein
KW - Lectin
KW - Pyridylamino derivative
KW - Ricinus communis agglutinin
KW - Sugar chain processing
KW - Sugar chain structure
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U2 - 10.1016/0304-4165(88)90118-3
DO - 10.1016/0304-4165(88)90118-3
M3 - Article
AN - SCOPUS:0000006124
SN - 0304-4165
VL - 966
SP - 248
EP - 256
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 2
ER -