Structures of Sugar Chains of Water-soluble Glycoproteins in Developing Castor Bean Cotyledons

Yoshinobu Kimura; Hiroshi Suehisa; Osamu Yamaguchi; Shuhei Nakajima; Shigeaki Takagi

doi:10.1271/bbb1961.54.3259

Structures of Sugar Chains of Water-soluble Glycoproteins in Developing Castor Bean Cotyledons

Yoshinobu Kimura, Hiroshi Suehisa, Osamu Yamaguchi, Shuhei Nakajima, Shigeaki Takagi

Research output: Contribution to journal › Article › peer-review

Abstract

The structures of sugar chains from the water-soluble glycoproteins in developing castor beans have been identified. The structural analyses were done by a fluorescence method combined with exoglycosidase digestions and¹H-NMR spectroscopy. The identified structures fell into two categories; one was an oligomannose-type, the other xylomannose-type or xylose-containing type. Among these oligosaccharides, Man₃Fuc₁Xyl₁GlcNAc₂(M3FX; 38%) and Man₆GlcNAc₂(M6B; 22%) were the major structures. The higher mannose-content oligosaccharides (Man_8-7GlcNAc₂) were only 4.1%, and the further-modified structures (_GNM3FX, M2FX) than M3FX were 22% of the total.

Original language	English
Pages (from-to)	3259-3267
Number of pages	9
Journal	Agricultural and Biological Chemistry
Volume	54
Issue number	12
DOIs	https://doi.org/10.1271/bbb1961.54.3259
Publication status	Published - 1990

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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@article{a235dbb113f04fbca320d8a4d91cafee,

title = "Structures of Sugar Chains of Water-soluble Glycoproteins in Developing Castor Bean Cotyledons",

abstract = "The structures of sugar chains from the water-soluble glycoproteins in developing castor beans have been identified. The structural analyses were done by a fluorescence method combined with exoglycosidase digestions and1H-NMR spectroscopy. The identified structures fell into two categories; one was an oligomannose-type, the other xylomannose-type or xylose-containing type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2(M3FX; 38%) and Man6GlcNAc2(M6B; 22%) were the major structures. The higher mannose-content oligosaccharides (Man8-7GlcNAc2) were only 4.1%, and the further-modified structures (GNM3FX, M2FX) than M3FX were 22% of the total.",

author = "Yoshinobu Kimura and Hiroshi Suehisa and Osamu Yamaguchi and Shuhei Nakajima and Shigeaki Takagi",

note = "Funding Information: t This work was supported in part by a Grant-in-Aid (No.Ol760075) for Scientific Research from the Ministry of Education, Science and Culture of Japan.",

year = "1990",

doi = "10.1271/bbb1961.54.3259",

language = "English",

volume = "54",

pages = "3259--3267",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "12",

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T1 - Structures of Sugar Chains of Water-soluble Glycoproteins in Developing Castor Bean Cotyledons

AU - Kimura, Yoshinobu

AU - Suehisa, Hiroshi

AU - Yamaguchi, Osamu

AU - Nakajima, Shuhei

AU - Takagi, Shigeaki

N1 - Funding Information: t This work was supported in part by a Grant-in-Aid (No.Ol760075) for Scientific Research from the Ministry of Education, Science and Culture of Japan.

PY - 1990

Y1 - 1990

N2 - The structures of sugar chains from the water-soluble glycoproteins in developing castor beans have been identified. The structural analyses were done by a fluorescence method combined with exoglycosidase digestions and1H-NMR spectroscopy. The identified structures fell into two categories; one was an oligomannose-type, the other xylomannose-type or xylose-containing type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2(M3FX; 38%) and Man6GlcNAc2(M6B; 22%) were the major structures. The higher mannose-content oligosaccharides (Man8-7GlcNAc2) were only 4.1%, and the further-modified structures (GNM3FX, M2FX) than M3FX were 22% of the total.

AB - The structures of sugar chains from the water-soluble glycoproteins in developing castor beans have been identified. The structural analyses were done by a fluorescence method combined with exoglycosidase digestions and1H-NMR spectroscopy. The identified structures fell into two categories; one was an oligomannose-type, the other xylomannose-type or xylose-containing type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2(M3FX; 38%) and Man6GlcNAc2(M6B; 22%) were the major structures. The higher mannose-content oligosaccharides (Man8-7GlcNAc2) were only 4.1%, and the further-modified structures (GNM3FX, M2FX) than M3FX were 22% of the total.

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JO - Bioscience, Biotechnology and Biochemistry

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IS - 12

ER -

Structures of Sugar Chains of Water-soluble Glycoproteins in Developing Castor Bean Cotyledons

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