Study of the interaction between actin and antitumor substance aplyronine A with a novel fluorescent photoaffinity probe

Takeshi Kuroda; Kiyotake Suenaga; Akira Sakakura; Tomohisa Handa; Kazuhito Okamoto; Hideo Kigoshi

doi:10.1021/bc050324i

Study of the interaction between actin and antitumor substance aplyronine A with a novel fluorescent photoaffinity probe

Takeshi Kuroda, Kiyotake Suenaga, Akira Sakakura, Tomohisa Handa, Kazuhito Okamoto, Hideo Kigoshi

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

The interaction between actin and aplyronine A, a potent antitumor and actin-depolymerizing substance of marine origin, was investigated by photoaffinity labeling experiments. Photoaffinity probes consisting of a side-chain portion of aplyronine A as a ligand, a diazirine moiety as a photoaffinity group, and a fluorophore as a detecting group were synthesized. Photolabeling experiments between actin and the probe were carried out. Actin was successfully photolabeled by the fluorescent probe and visualized clearly. The present results provide the first chemical evidence for the direct interaction between actin and the side-chain portion of aplyronine A.

Original language	English
Pages (from-to)	524-529
Number of pages	6
Journal	Bioconjugate Chemistry
Volume	17
Issue number	2
DOIs	https://doi.org/10.1021/bc050324i
Publication status	Published - Mar 1 2006
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/bc050324i

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abstract = "The interaction between actin and aplyronine A, a potent antitumor and actin-depolymerizing substance of marine origin, was investigated by photoaffinity labeling experiments. Photoaffinity probes consisting of a side-chain portion of aplyronine A as a ligand, a diazirine moiety as a photoaffinity group, and a fluorophore as a detecting group were synthesized. Photolabeling experiments between actin and the probe were carried out. Actin was successfully photolabeled by the fluorescent probe and visualized clearly. The present results provide the first chemical evidence for the direct interaction between actin and the side-chain portion of aplyronine A.",

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AU - Kuroda, Takeshi

AU - Suenaga, Kiyotake

AU - Sakakura, Akira

AU - Handa, Tomohisa

AU - Okamoto, Kazuhito

AU - Kigoshi, Hideo

PY - 2006/3/1

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N2 - The interaction between actin and aplyronine A, a potent antitumor and actin-depolymerizing substance of marine origin, was investigated by photoaffinity labeling experiments. Photoaffinity probes consisting of a side-chain portion of aplyronine A as a ligand, a diazirine moiety as a photoaffinity group, and a fluorophore as a detecting group were synthesized. Photolabeling experiments between actin and the probe were carried out. Actin was successfully photolabeled by the fluorescent probe and visualized clearly. The present results provide the first chemical evidence for the direct interaction between actin and the side-chain portion of aplyronine A.

AB - The interaction between actin and aplyronine A, a potent antitumor and actin-depolymerizing substance of marine origin, was investigated by photoaffinity labeling experiments. Photoaffinity probes consisting of a side-chain portion of aplyronine A as a ligand, a diazirine moiety as a photoaffinity group, and a fluorophore as a detecting group were synthesized. Photolabeling experiments between actin and the probe were carried out. Actin was successfully photolabeled by the fluorescent probe and visualized clearly. The present results provide the first chemical evidence for the direct interaction between actin and the side-chain portion of aplyronine A.

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Study of the interaction between actin and antitumor substance aplyronine A with a novel fluorescent photoaffinity probe

Abstract

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