TY - JOUR
T1 - Study on the effects of chloride depletion on photosystem II using different chloride depletion methods
AU - Jajoo, Anjana
AU - Mathur, Sonal
AU - Mehta, Pooja
AU - Yoshoika, Miho
AU - Allakhverdiev, Suleyman
AU - Yamamoto, Yasusi
N1 - Funding Information:
Acknowledgments A short term overseas fellowship of Department of Biotechnology (DBT), India to AJ to visit Okayama University (Japan) is thankfully acknowledged. The project from DST (INT/ ILTP/B.6–27) to AJ is thankfully acknowledged. This work was supported, in part, by grants from Russian Foundation for Basic Research (08-04-00241 and 09-04-91219-CT) and from the Molecular and Cellular Biology Programs of the Russian Academy of Sciences (to SIA).
PY - 2010/2
Y1 - 2010/2
N2 - Chloride is an indispensable factor for the functioning of oxygen evolving complex (OEC) and has protective and activating effects on photosystem II. In this study we have investigated mainly by EPR, the properties of chloride-sufficient, chloride-deficient and chloride-depleted thylakoid membranes and photosystem II enriched membranes from spinach. The results on the effects of different chloride depletion methods on the structural and functional aspects of photosystem II showed that chloride-depletion by treating PS II membranes with high pH is a relatively harsh way causing a significant and irreparable damage to the PS II donor side. Damage to the acceptor side of PS II was recovered almost fully in chloride-deficient as well as chloride-depleted PS II membranes.
AB - Chloride is an indispensable factor for the functioning of oxygen evolving complex (OEC) and has protective and activating effects on photosystem II. In this study we have investigated mainly by EPR, the properties of chloride-sufficient, chloride-deficient and chloride-depleted thylakoid membranes and photosystem II enriched membranes from spinach. The results on the effects of different chloride depletion methods on the structural and functional aspects of photosystem II showed that chloride-depletion by treating PS II membranes with high pH is a relatively harsh way causing a significant and irreparable damage to the PS II donor side. Damage to the acceptor side of PS II was recovered almost fully in chloride-deficient as well as chloride-depleted PS II membranes.
KW - Chloride
KW - EPR
KW - Oxygen evolving complex
KW - Photosystem II
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U2 - 10.1007/s10863-009-9260-4
DO - 10.1007/s10863-009-9260-4
M3 - Article
C2 - 20054625
AN - SCOPUS:77950019798
SN - 0145-479X
VL - 42
SP - 47
EP - 53
JO - Journal of Bioenergetics and Biomembranes
JF - Journal of Bioenergetics and Biomembranes
IS - 1
ER -