TY - JOUR
T1 - Study on the metabolites of isoalantolactone in vivo and in vitro by ultra performance liquid chromatography combined with Triple TOF mass spectrometry
AU - Yao, Donggui
AU - Wang, Yufang
AU - Huo, Changhong
AU - Wu, Yibing
AU - Zhang, Manli
AU - Li, Ligeng
AU - Shi, Qingwen
AU - Kiyota, Hiromasa
AU - Shi, Xiaowei
N1 - Funding Information:
This work was supported by grants from the National Program on Key Basic Research Project of China (973 Program, No. 2012CB518601 ), Natural Science Foundation of Hebei Province ( H2015206113 ), the Project of Department of Education , Hebei Province ( 291090501 ).
Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Isoalantolactone (IAL) is an active sesquiterpene naturally present in many vegetables, condiments and medicinal plants. In this study, an efficient strategy was developed for the identification of metabolites following the in vivo metabolism and in vitro biotransformation of IAL with rat intestinal bacteria utilizing ultra performance liquid chromatography combined with Triple TOF mass spectrometry (UPLC-Triple TOF-MS/MS). As a result, 46 metabolites including 34 novel sulfur-containing products were identified. The results demonstrated that IAL could undergo general metabolic reactions, including oxidation, hydration, hydrogenation, demethylation, cysteine conjugation and N-acetylcysteine conjugation, but the most noticeable characteristic of IAL metabolism was the H2S addition to the double bond between C-11 and C-13 and subsequent reactions to produce a series of novel sulfur-containing dimers. This is the first study of IAL metabolism in vivo, whose results provide novel and useful data for a better understanding of the safety and efficacy of IAL.
AB - Isoalantolactone (IAL) is an active sesquiterpene naturally present in many vegetables, condiments and medicinal plants. In this study, an efficient strategy was developed for the identification of metabolites following the in vivo metabolism and in vitro biotransformation of IAL with rat intestinal bacteria utilizing ultra performance liquid chromatography combined with Triple TOF mass spectrometry (UPLC-Triple TOF-MS/MS). As a result, 46 metabolites including 34 novel sulfur-containing products were identified. The results demonstrated that IAL could undergo general metabolic reactions, including oxidation, hydration, hydrogenation, demethylation, cysteine conjugation and N-acetylcysteine conjugation, but the most noticeable characteristic of IAL metabolism was the H2S addition to the double bond between C-11 and C-13 and subsequent reactions to produce a series of novel sulfur-containing dimers. This is the first study of IAL metabolism in vivo, whose results provide novel and useful data for a better understanding of the safety and efficacy of IAL.
KW - Identification
KW - Intestinal bacteria biotransformation
KW - Isoalantolactone
KW - Metabolites
KW - UPLC-Triple TOF-MS/MS
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U2 - 10.1016/j.foodchem.2016.07.100
DO - 10.1016/j.foodchem.2016.07.100
M3 - Article
C2 - 27507482
AN - SCOPUS:84978437338
SN - 0308-8146
VL - 214
SP - 328
EP - 338
JO - Food Chemistry
JF - Food Chemistry
ER -