Studying plant salt tolerance with the voltage clamp technique

Zhong Hua Chen, Dezhi Wu, Cornelia Eisenach, Adrian Hills, Guoping Zhang, Michael R. Blatt

    Research output: Chapter in Book/Report/Conference proceedingChapter


    Voltage clamp is one of the key techniques for the dissection, identification, and monitoring of ion transporters in plant cells. Voltage clamp-based research work on salinity stress in plants enables the characterization of many plant ATP-dependent pumps, ion channels, and ion-coupled carriers through heterologous expression in Xenopus laevis oocytes and in vivo measurements in salt-tolerant and salt-sensitive giant green algae such as Chara and many plant species. We have modified and developed a reliable set of procedures for voltage clamp analysis in intact guard cells and root epidermal cells from Arabidopsis thaliana with potentially broad applications in the salinity response of plants. These procedures greatly extend the duration of measurements and scope for analysis of the predominant K+ and anion channels.

    Original languageEnglish
    Title of host publicationPlant Salt Tolerance
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press Inc.
    Number of pages15
    ISBN (Print)9781617799853
    Publication statusPublished - Jan 1 2012

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745


    • Arabidopsis thaliana
    • Guard cell
    • Microelectrode
    • Root epidermal cell
    • Voltage clamp

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics


    Dive into the research topics of 'Studying plant salt tolerance with the voltage clamp technique'. Together they form a unique fingerprint.

    Cite this