TY - JOUR
T1 - Subretinal implantation of Okayama university-type retinal prosthesis (OURepTM) in canine eyes by vitrectomy
AU - Matsuo, Toshihiko
AU - Uchida, Tetsuya
AU - Nitta, Makoto
AU - Yamashita, Koichiro
AU - Takei, Shigiko
AU - Ido, Daisuke
AU - Tanaka, Mamoru
AU - Oguchi, Masao
AU - Furukawa, Toshinori
N1 - Funding Information:
This study was supported by a grant for the Translational Research Network Program from the Japan Agency for Medical Research and Development (AMED). We thank Chie Matsuo, DDS, PhD, for preparation of figures.
Publisher Copyright:
© 2017 The Japanese Society of Veterinary Science.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Okayama University-type retinal prosthesis (OUReP™) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility of subretinal implantation and functional durability of dye-coupled films in the subretinal space. The dye-coupled films were implanted subretinally by 25-gauge vitrectomy in the right eye of 11 normal beagle dogs: 2 dogs served for film removal after 5-month film implantation, 3 dogs for film removal after 3-month film implantation, 3 dogs for 3-month film implantation and pathological examination, and 3 dogs for sham surgery. The surface electric potential of the removed dye-coupled films in response to light was measured by the Kelvin Probe system. At surgery, rolled-up dye-coupled films in 5 × 5 mm square size could be inserted into subretinal space of retinal detachment induced by fluid injection with a 38-gauge polyimide tip. Retinal attachment was maintained by silicone oil injection in vitreous cavity. At autopsy, the retina in all dogs maintained the ganglion cell layer, inner and outer nuclear layers while it lost the outer segments in some part. All 5 sheets of removed dye-coupled films maintained the dye color. One sheet of the 5-month implanted film showed proportional increase of surface potential in response to increasing light intensity. Subretinal implantation of OUReP™ by vitrectomy was technically feasible in canine eyes, and OUReP™ maintained the function of generating light-evoked surface potential after 5 months in subretinal implantation.
AB - Okayama University-type retinal prosthesis (OUReP™) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility of subretinal implantation and functional durability of dye-coupled films in the subretinal space. The dye-coupled films were implanted subretinally by 25-gauge vitrectomy in the right eye of 11 normal beagle dogs: 2 dogs served for film removal after 5-month film implantation, 3 dogs for film removal after 3-month film implantation, 3 dogs for 3-month film implantation and pathological examination, and 3 dogs for sham surgery. The surface electric potential of the removed dye-coupled films in response to light was measured by the Kelvin Probe system. At surgery, rolled-up dye-coupled films in 5 × 5 mm square size could be inserted into subretinal space of retinal detachment induced by fluid injection with a 38-gauge polyimide tip. Retinal attachment was maintained by silicone oil injection in vitreous cavity. At autopsy, the retina in all dogs maintained the ganglion cell layer, inner and outer nuclear layers while it lost the outer segments in some part. All 5 sheets of removed dye-coupled films maintained the dye color. One sheet of the 5-month implanted film showed proportional increase of surface potential in response to increasing light intensity. Subretinal implantation of OUReP™ by vitrectomy was technically feasible in canine eyes, and OUReP™ maintained the function of generating light-evoked surface potential after 5 months in subretinal implantation.
KW - 38-gauge polyimide tip
KW - Dog
KW - Dye-coupled thin film retinal prosthesis
KW - Photoelectric dye
KW - Vitrectomy
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U2 - 10.1292/jvms.17-0450
DO - 10.1292/jvms.17-0450
M3 - Article
C2 - 29046507
AN - SCOPUS:85040053783
SN - 0916-7250
VL - 79
SP - 1939
EP - 1946
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 12
ER -