TY - JOUR
T1 - Subsite Affinities of α-Glucosidases from Spinach Seeds
AU - Furui, Satoshi
AU - Sugimoto, Manabu
AU - Suzuki, Yukio
N1 - Funding Information:
t This work was supported in part by a Grant-in-Aid for Science Research from the Ministry of Education, Science, and Culture of Japan, and by a grant of the Ohara Foundation in Kurashiki with the purpose of promoting the advancement of agricultural sciences. tt Author to whom corrcspondence should be addressed.
PY - 1997/1
Y1 - 1997/1
N2 - The rate parameters for maltooligosaccharides of two α-glucosidases from spinach seeds were examined and subsite affinities were evaluated. The subsite affinities for subsites 1, 2, 3, 4, 5, 6, and 7 in the active site of α-glucosidase A and B were 1.73, 2.91, 1.10, 0.25, 0.94, 0.03, and −0.01 kcal/mol, and 0.33, 4.94, 0.26, 0.14, −0.24, −0.52, and 0.14 kcal/mol, respectively. The six and four subsites exist in α-glucosidase A and B, respectively, which maltooligosaccharides or soluble starch could be bound.
AB - The rate parameters for maltooligosaccharides of two α-glucosidases from spinach seeds were examined and subsite affinities were evaluated. The subsite affinities for subsites 1, 2, 3, 4, 5, 6, and 7 in the active site of α-glucosidase A and B were 1.73, 2.91, 1.10, 0.25, 0.94, 0.03, and −0.01 kcal/mol, and 0.33, 4.94, 0.26, 0.14, −0.24, −0.52, and 0.14 kcal/mol, respectively. The six and four subsites exist in α-glucosidase A and B, respectively, which maltooligosaccharides or soluble starch could be bound.
KW - Multiple molecular forms
KW - Spinach seeds
KW - Subsite affinity
KW - α-glucosidase
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U2 - 10.1271/bbb.61.1382
DO - 10.1271/bbb.61.1382
M3 - Article
AN - SCOPUS:85009548526
SN - 0916-8451
VL - 61
SP - 1382
EP - 1383
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 8
ER -