Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia

Yuji Endo, Yoshitaka Fujii, Shozo Kurotsuchi, Hiroaki Motoyama, Hiroaki Funahashi

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Objective: To report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with vitrified sperm from patients with severe male factor infertility. Design: Retrospective case series. Setting: IVF unit of a medical center. Patient(s): Three patients with severe oligozoospermia or nonobstructive azoospermia (NOA). Intervention(s): Cryopreservation of limited numbers of spermatozoa with the use of Cryotop and Cell Sleeper as nonbiologic containers. Main Outcome Measure(s): Four cycles underwent intracytoplasmic sperm injection (ICSI) with vitrified sperm. Result(s): A total of 148 spermatozoa in 18 containers (8.2 sperm per container) were vitrified and 36 of them (5 containers) were warmed. Thirty-three sperm (92%) were retrieved successfully and injected individually into 17 mature oocytes. Fertilization was observed in 12 oocytes (71%), and all zygotes (100%) cleaved. A couple with NOA achieved a singleton pregnancy and concluded with full-term delivery of a healthy boy (2,632 g). Conclusion(s): A successful delivery was achieved after transfer of a blastocyst derived from vitrified-warmed spermatozoa. A small number of vitrified sperm cells were used for ICSI to fertilize oocytes with predictable timing.

Original languageEnglish
Pages (from-to)1423-1427
Number of pages5
JournalFertility and Sterility
Issue number6
Publication statusPublished - Dec 2012


  • Cell Sleeper
  • Cryotop
  • Severe male factor infertility
  • live birth
  • single sperm vitrification

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology


Dive into the research topics of 'Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia'. Together they form a unique fingerprint.

Cite this