1H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity

Nwe Haymar Lynn; Thein Zaw Linn; Cui Yanmei; Yuki Shimozu; Shoko Taniguchi; Tsutomu Hatano

doi:10.1080/09168451.2019.1569493

¹H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity

Nwe Haymar Lynn, Thein Zaw Linn, Cui Yanmei, Yuki Shimozu, Shoko Taniguchi, Tsutomu Hatano

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46-64% inhibition at 1.0 mg/mL), despite the high content (46-64%) of β-asarone, which independently had a potent effect (IC₅₀ 2.9 µM [0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone.

Original language	English
Pages (from-to)	892-900
Number of pages	9
Journal	Bioscience, Biotechnology and Biochemistry
Volume	83
Issue number	5
DOIs	https://doi.org/10.1080/09168451.2019.1569493
Publication status	Published - 2019

Keywords

Acetylcholine esterase inhibitory activity
Acorus
Eudesmin A
QNMR
β-asarone

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Lynn, N. H., Linn, T. Z., Yanmei, C., Shimozu, Y., Taniguchi, S., & Hatano, T. (2019). ¹H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity. Bioscience, Biotechnology and Biochemistry, 83(5), 892-900. https://doi.org/10.1080/09168451.2019.1569493

¹H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity. / Lynn, Nwe Haymar; Linn, Thein Zaw; Yanmei, Cui et al.
In: Bioscience, Biotechnology and Biochemistry, Vol. 83, No. 5, 2019, p. 892-900.

Research output: Contribution to journal › Article › peer-review

Lynn, NH, Linn, TZ, Yanmei, C, Shimozu, Y, Taniguchi, S & Hatano, T 2019, '¹H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity', Bioscience, Biotechnology and Biochemistry, vol. 83, no. 5, pp. 892-900. https://doi.org/10.1080/09168451.2019.1569493

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abstract = "We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46-64% inhibition at 1.0 mg/mL), despite the high content (46-64%) of β-asarone, which independently had a potent effect (IC50 2.9 µM [0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone.",

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AU - Shimozu, Yuki

AU - Taniguchi, Shoko

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