TY - JOUR
T1 - Suppression of immunorejection against rat fetal dopaminergic neurons in a mouse brain by 15-deoxyspergualin
AU - Zhou, Jiawei
AU - Date, Isao
AU - Sakai, Kyoji
AU - Yoshimoto, Yusuke
AU - Furuta, Tomohisa
AU - Asari, Shoji
AU - Ohmoto, Takashi
N1 - Funding Information:
We thank Mr. Hideki Wakimoto for the photography. This work was supported in part by grant from the Ministry of Education, Science and Culture, Japan, 04770989 to I.D.
PY - 1993/9/3
Y1 - 1993/9/3
N2 - In this study, the immunosuppressive effect of 15-deoxyspergualin (DSG) on the survivability of rat embryonic dopaminergic neurons grafted into the lateral ventricle of adult mice was investigated. DSG was administered daily in dose of 5 mg/kg for 2 weeks postgrafting, commencing on the day of transplantation, in the immunosuppressant-treated groups. Animals were then allowed to survive for 2 to 4 weeks after transplantation. Histological analysis revealed that most of transplanted rat fetal tissue was destroyed and scavenged in 2 weeks after transplantation in the non-immunosuppressed group. However, a large number of tyrosine hydroxylase (TH)-positive grafted neurons survived and grew postgrafting in the brain of the host administered with DSG even if they suffered from T lymphocyte infiltrations visualized by cytotoxic T cell immunohistochemistry. The results thus indicated that DSG is an potent immunosuppressant in neural transplantation as well as in transplantation of other organs in animals. It seems to be able to block, at least in part, the ability of mature specific cytotoxic T cells to lyse their target.
AB - In this study, the immunosuppressive effect of 15-deoxyspergualin (DSG) on the survivability of rat embryonic dopaminergic neurons grafted into the lateral ventricle of adult mice was investigated. DSG was administered daily in dose of 5 mg/kg for 2 weeks postgrafting, commencing on the day of transplantation, in the immunosuppressant-treated groups. Animals were then allowed to survive for 2 to 4 weeks after transplantation. Histological analysis revealed that most of transplanted rat fetal tissue was destroyed and scavenged in 2 weeks after transplantation in the non-immunosuppressed group. However, a large number of tyrosine hydroxylase (TH)-positive grafted neurons survived and grew postgrafting in the brain of the host administered with DSG even if they suffered from T lymphocyte infiltrations visualized by cytotoxic T cell immunohistochemistry. The results thus indicated that DSG is an potent immunosuppressant in neural transplantation as well as in transplantation of other organs in animals. It seems to be able to block, at least in part, the ability of mature specific cytotoxic T cells to lyse their target.
KW - 15-Deoxyspergualin
KW - Cross-species
KW - Cytotoxic T cell
KW - Immunosuppressant
KW - Neural transplantation
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U2 - 10.1016/0006-8993(93)90313-C
DO - 10.1016/0006-8993(93)90313-C
M3 - Article
C2 - 8221068
AN - SCOPUS:0027308107
SN - 0006-8993
VL - 621
SP - 155
EP - 160
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -