TY - JOUR
T1 - Surface-Engineered Design of Efficient Luminescent Europium(III) Complex-Based Hydroxyapatite Nanocrystals for Rapid HeLa Cancer Cell Imaging
AU - Kataoka, Takuya
AU - Abe, Shigeaki
AU - Tagaya, Motohiro
N1 - Funding Information:
This study was partially supported by a grant from the Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant-in-Aid for Young Scientists (A), Grant No. 17H04954, and Grant-in-Aid for JSPS Fellows, Grant No. 18J20271). The authors acknowledge Analysis and Instrumentation Center in Nagaoka University of Technology for providing the facilities.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/3/6
Y1 - 2019/3/6
N2 - We synthesized hydroxyapatite nanocrystals under the existence of tris(2,2,6,6-tetramethyl-3,5-heptanedionato)europium(III) (EuTH) complex to form inorganic/organic hybrid nanocrystal (EHA). Then, the folic acid derivative (folate N-hydroxysuccinimidyl ester (FA-NHS)) as the targeting ligand for the HeLa cancer cells was immobilized on the EHA by the mediation of both 3-aminopropyltriethoxysilane and methyltriethoxysilane molecules. Here, we investigated the photofunctions based on the interfacial interactions between the FA-NHS and EHA nanohybrids for preparing the novel bioimaging nanomaterials. As a result, the photofunctions could be changed by the FA-NHS molecular occupancy on the EHA. When the molecular occupancy ratio to the EHA surfaces is at around 3-5%, the intense luminescence from the f-f transition of the Eu 3+ ions as well as the charge transfer between the EuTH-FA-NHS was observed to exhibit higher quantum efficiency. Moreover, effective dispersibility in phosphate-buffered saline was confirmed with immobilizing the positively charged FA-NHS. The cytotoxicity against the HeLa cells was also evaluated to verify whether the nanohybrids can be the candidate for cell imaging. The affinity and noncytotoxicity between the FA-NHS-immobilized EHA nanohybrids and cells were monitored for 3 days. Red luminescence from the cells could be observed, and the labels with following the cellular shapes were achieved by an additional culture time of 1 h after injecting the FA-NHS-immobilized EHA nanohybrids to the spheres, indicating the rapid bioimaging process. Therefore, this is the first successful report to describe the synthesis of inorganic-organic nanohybrid systems for controlling the EuTH-FA-NHS interactions. The photofunction of the interfacial interactions was successfully designed to provide "efficient luminescent ability" as well as "rapid targeting to the cancer cells" in one particle.
AB - We synthesized hydroxyapatite nanocrystals under the existence of tris(2,2,6,6-tetramethyl-3,5-heptanedionato)europium(III) (EuTH) complex to form inorganic/organic hybrid nanocrystal (EHA). Then, the folic acid derivative (folate N-hydroxysuccinimidyl ester (FA-NHS)) as the targeting ligand for the HeLa cancer cells was immobilized on the EHA by the mediation of both 3-aminopropyltriethoxysilane and methyltriethoxysilane molecules. Here, we investigated the photofunctions based on the interfacial interactions between the FA-NHS and EHA nanohybrids for preparing the novel bioimaging nanomaterials. As a result, the photofunctions could be changed by the FA-NHS molecular occupancy on the EHA. When the molecular occupancy ratio to the EHA surfaces is at around 3-5%, the intense luminescence from the f-f transition of the Eu 3+ ions as well as the charge transfer between the EuTH-FA-NHS was observed to exhibit higher quantum efficiency. Moreover, effective dispersibility in phosphate-buffered saline was confirmed with immobilizing the positively charged FA-NHS. The cytotoxicity against the HeLa cells was also evaluated to verify whether the nanohybrids can be the candidate for cell imaging. The affinity and noncytotoxicity between the FA-NHS-immobilized EHA nanohybrids and cells were monitored for 3 days. Red luminescence from the cells could be observed, and the labels with following the cellular shapes were achieved by an additional culture time of 1 h after injecting the FA-NHS-immobilized EHA nanohybrids to the spheres, indicating the rapid bioimaging process. Therefore, this is the first successful report to describe the synthesis of inorganic-organic nanohybrid systems for controlling the EuTH-FA-NHS interactions. The photofunction of the interfacial interactions was successfully designed to provide "efficient luminescent ability" as well as "rapid targeting to the cancer cells" in one particle.
KW - bioimaging particles
KW - cancer cell imaging
KW - europium(III) complex
KW - hydroxyapatite nanocrystals
KW - inorganic-organic nanohybrid systems
KW - luminescent nanomaterials
KW - photofunctional interfaces
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UR - http://www.scopus.com/inward/citedby.url?scp=85062362015&partnerID=8YFLogxK
U2 - 10.1021/acsami.8b22740
DO - 10.1021/acsami.8b22740
M3 - Article
C2 - 30730134
AN - SCOPUS:85062362015
SN - 1944-8244
VL - 11
SP - 8915
EP - 8927
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 9
ER -
