Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor

Shuji Tanaka; Elizabeth A. Robinson; Teizo Yoshimura; Kouji Matsushima; Edward J. Leonard; Ettore Appella

doi:10.1016/0014-5793(88)80078-4

Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor

Shuji Tanaka, Elizabeth A. Robinson, Teizo Yoshimura, Kouji Matsushima, Edward J. Leonard, Ettore Appella

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.

Original language	English
Pages (from-to)	467-470
Number of pages	4
Journal	FEBS Letters
Volume	236
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(88)80078-4
Publication status	Published - Aug 29 1988

Keywords

Neutrophil chemotaxis
Peptide synthesis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(88)80078-4

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title = "Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor",

abstract = "MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.",

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author = "Shuji Tanaka and Robinson, {Elizabeth A.} and Teizo Yoshimura and Kouji Matsushima and Leonard, {Edward J.} and Ettore Appella",

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T1 - Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor

AU - Tanaka, Shuji

AU - Robinson, Elizabeth A.

AU - Yoshimura, Teizo

AU - Matsushima, Kouji

AU - Leonard, Edward J.

AU - Appella, Ettore

PY - 1988/8/29

Y1 - 1988/8/29

N2 - MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.

AB - MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.

KW - Neutrophil chemotaxis

KW - Peptide synthesis

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Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor

Abstract

Keywords

ASJC Scopus subject areas

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