Synthesis of glyceryl ferulate by immobilized ferulic acid esterase

Takemasa Matsuo; Takashi Kobayashi; Yukitaka Kimura; Moriyasu Tsuchiyama; Tadanobu Oh; Tatsuji Sakamoto; Shuji Adachi

doi:10.1007/s10529-008-9814-2

Synthesis of glyceryl ferulate by immobilized ferulic acid esterase

Takemasa Matsuo, Takashi Kobayashi, Yukitaka Kimura, Moriyasu Tsuchiyama, Tadanobu Oh, Tatsuji Sakamoto, Shuji Adachi

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50°C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25°C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.

Original language	English
Pages (from-to)	2151-2156
Number of pages	6
Journal	Biotechnology Letters
Volume	30
Issue number	12
DOIs	https://doi.org/10.1007/s10529-008-9814-2
Publication status	Published - Dec 1 2008
Externally published	Yes

Keywords

Ferulic acid
Ferulic acid esterase
Glyceryl ferulate
Immobilized enzyme
Repeated batch reaction

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1007/s10529-008-9814-2

Cite this

@article{f3b82f6a71ad41eb818095d6ee2417c9,

title = "Synthesis of glyceryl ferulate by immobilized ferulic acid esterase",

abstract = "Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL {"}Amano{"} from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50°C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25°C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.",

keywords = "Ferulic acid, Ferulic acid esterase, Glyceryl ferulate, Immobilized enzyme, Repeated batch reaction",

author = "Takemasa Matsuo and Takashi Kobayashi and Yukitaka Kimura and Moriyasu Tsuchiyama and Tadanobu Oh and Tatsuji Sakamoto and Shuji Adachi",

year = "2008",

month = dec,

day = "1",

doi = "10.1007/s10529-008-9814-2",

language = "English",

volume = "30",

pages = "2151--2156",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "12",

}

TY - JOUR

T1 - Synthesis of glyceryl ferulate by immobilized ferulic acid esterase

AU - Matsuo, Takemasa

AU - Kobayashi, Takashi

AU - Kimura, Yukitaka

AU - Tsuchiyama, Moriyasu

AU - Oh, Tadanobu

AU - Sakamoto, Tatsuji

AU - Adachi, Shuji

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50°C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25°C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.

AB - Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50°C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25°C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.

KW - Ferulic acid

KW - Ferulic acid esterase

KW - Glyceryl ferulate

KW - Immobilized enzyme

KW - Repeated batch reaction

UR - http://www.scopus.com/inward/record.url?scp=54849417360&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54849417360&partnerID=8YFLogxK

U2 - 10.1007/s10529-008-9814-2

DO - 10.1007/s10529-008-9814-2

M3 - Article

C2 - 18688576

AN - SCOPUS:54849417360

SN - 0141-5492

VL - 30

SP - 2151

EP - 2156

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 12

ER -

Synthesis of glyceryl ferulate by immobilized ferulic acid esterase

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this