Synthesis of hepatitis B virus e antigen in E. coli

Takashi Inada; Yuko Misumi; Masaharu Seno; Shuichi Kanezaki; Yasuo Shibata; Yushi Oka; Haruo Onda

doi:10.1016/0168-1702(89)90067-1

Synthesis of hepatitis B virus e antigen in E. coli

Takashi Inada, Yuko Misumi, Masaharu Seno, Shuichi Kanezaki, Yasuo Shibata, Yushi Oka, Haruo Onda

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Hepatitis B virus core antigen (HBcAg) gene was deleted at some unique restriction enzyme sites, or at random, and inserted into the expression plasmids of E. coli which had the tryptophan promoter. E. coli transformants with the plasmids, synthesized materials with many kinds of antigenicity of HBcAg, HBeAg, or both HBcAg and HBeAg. HBeAg-specific material smaller than native HBeAg was produced in a stable condition.

Original language	English
Pages (from-to)	27-47
Number of pages	21
Journal	Virus research
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/0168-1702(89)90067-1
Publication status	Published - Sept 1989
Externally published	Yes

Keywords

E. coli
Gene expression
HBcAg and HBeAg
HBeAg specific polypeptide
Hepatitis B virus

ASJC Scopus subject areas

Cancer Research
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0168-1702(89)90067-1

Cite this

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title = "Synthesis of hepatitis B virus e antigen in E. coli",

abstract = "Hepatitis B virus core antigen (HBcAg) gene was deleted at some unique restriction enzyme sites, or at random, and inserted into the expression plasmids of E. coli which had the tryptophan promoter. E. coli transformants with the plasmids, synthesized materials with many kinds of antigenicity of HBcAg, HBeAg, or both HBcAg and HBeAg. HBeAg-specific material smaller than native HBeAg was produced in a stable condition.",

keywords = "E. coli, Gene expression, HBcAg and HBeAg, HBeAg specific polypeptide, Hepatitis B virus",

author = "Takashi Inada and Yuko Misumi and Masaharu Seno and Shuichi Kanezaki and Yasuo Shibata and Yushi Oka and Haruo Onda",

year = "1989",

month = sep,

doi = "10.1016/0168-1702(89)90067-1",

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pages = "27--47",

journal = "Virus research",

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T1 - Synthesis of hepatitis B virus e antigen in E. coli

AU - Inada, Takashi

AU - Misumi, Yuko

AU - Seno, Masaharu

AU - Kanezaki, Shuichi

AU - Shibata, Yasuo

AU - Oka, Yushi

AU - Onda, Haruo

PY - 1989/9

Y1 - 1989/9

N2 - Hepatitis B virus core antigen (HBcAg) gene was deleted at some unique restriction enzyme sites, or at random, and inserted into the expression plasmids of E. coli which had the tryptophan promoter. E. coli transformants with the plasmids, synthesized materials with many kinds of antigenicity of HBcAg, HBeAg, or both HBcAg and HBeAg. HBeAg-specific material smaller than native HBeAg was produced in a stable condition.

AB - Hepatitis B virus core antigen (HBcAg) gene was deleted at some unique restriction enzyme sites, or at random, and inserted into the expression plasmids of E. coli which had the tryptophan promoter. E. coli transformants with the plasmids, synthesized materials with many kinds of antigenicity of HBcAg, HBeAg, or both HBcAg and HBeAg. HBeAg-specific material smaller than native HBeAg was produced in a stable condition.

KW - E. coli

KW - Gene expression

KW - HBcAg and HBeAg

KW - HBeAg specific polypeptide

KW - Hepatitis B virus

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DO - 10.1016/0168-1702(89)90067-1

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C2 - 2683475

AN - SCOPUS:0024425023

SN - 0168-1702

VL - 14

SP - 27

EP - 47

JO - Virus research

JF - Virus research

IS - 1

ER -

Synthesis of hepatitis B virus e antigen in E. coli

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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