Systematic deletion of rRNAs for investigating ribosome architecture and function.

Kei Kitahara; Neuza S. Sato; Naoki Namba; Takuya Yokota; Tarou Tsujimura; Tsutomu Suzuki

doi:10.1093/nass/nrl143

Systematic deletion of rRNAs for investigating ribosome architecture and function.

Kei Kitahara, Neuza S. Sato, Naoki Namba, Takuya Yokota, Tarou Tsujimura, Tsutomu Suzuki

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Ribosomal RNAs (rRNAs) form a fundamental structure of ribosome, and play crucial roles in protein biosynthesis. To investigate functional importance of RNA helices in both 16S and 23S rRNAs, we devised an E. coli genetic system that allowed us to identify and select functional deletions in rRNAs. We here describe many RNA helices in rRNAs to be shortened or completely truncated. These results suggest architectural flexibility of rRNAs in ribosome assembly and function.

Original language	English
Pages (from-to)	287-288
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	50
DOIs	https://doi.org/10.1093/nass/nrl143
Publication status	Published - 2006
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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10.1093/nass/nrl143

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title = "Systematic deletion of rRNAs for investigating ribosome architecture and function.",

abstract = "Ribosomal RNAs (rRNAs) form a fundamental structure of ribosome, and play crucial roles in protein biosynthesis. To investigate functional importance of RNA helices in both 16S and 23S rRNAs, we devised an E. coli genetic system that allowed us to identify and select functional deletions in rRNAs. We here describe many RNA helices in rRNAs to be shortened or completely truncated. These results suggest architectural flexibility of rRNAs in ribosome assembly and function.",

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AU - Kitahara, Kei

AU - Sato, Neuza S.

AU - Namba, Naoki

AU - Yokota, Takuya

AU - Tsujimura, Tarou

AU - Suzuki, Tsutomu

PY - 2006

Y1 - 2006

N2 - Ribosomal RNAs (rRNAs) form a fundamental structure of ribosome, and play crucial roles in protein biosynthesis. To investigate functional importance of RNA helices in both 16S and 23S rRNAs, we devised an E. coli genetic system that allowed us to identify and select functional deletions in rRNAs. We here describe many RNA helices in rRNAs to be shortened or completely truncated. These results suggest architectural flexibility of rRNAs in ribosome assembly and function.

AB - Ribosomal RNAs (rRNAs) form a fundamental structure of ribosome, and play crucial roles in protein biosynthesis. To investigate functional importance of RNA helices in both 16S and 23S rRNAs, we devised an E. coli genetic system that allowed us to identify and select functional deletions in rRNAs. We here describe many RNA helices in rRNAs to be shortened or completely truncated. These results suggest architectural flexibility of rRNAs in ribosome assembly and function.

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JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

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Systematic deletion of rRNAs for investigating ribosome architecture and function.

Abstract

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