Tardive decrease of astrocytic glutamate transporter protein in transgenic mice with ALS-linked mutant SOD1

H. Warita; Y. Manabe; T. Murakami; M. Shiote; Y. Shiro; T. Hayashi; I. Nagano; M. Shoji; K. Abe

doi:10.1179/016164102101200384

Tardive decrease of astrocytic glutamate transporter protein in transgenic mice with ALS-linked mutant SOD1

H. Warita, Y. Manabe, T. Murakami, M. Shiote, Y. Shiro, T. Hayashi, I. Nagano, M. Shoji, K. Abe

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The expressions of glutamate transporter proteins were immunocytochemically examined in the spinal cord of transgenic mice harboring a Gly⁹³ → Ala (G93A) mutant human SOD1 gene. Astroglial EAAT2 protein level was preserved in the ventral horn even after the beginning of paralysis, and finally decreased at terminal stage of the disease (35 weeks of age), when neuronal EAAT3 protein level was also decreased. In contrast, glial fibrillary acidic protein (GFAP) immunoreactivity progressively increased from 25 weeks of age in the ventral horn. The present results show interesting dissociative expressions of astroglial proteins EAAT2 and GFAP in the same ventral horn, but suggest not an early and primary role of EAAT2 in the motoneuronal death of this model.

Original language	English
Pages (from-to)	577-581
Number of pages	5
Journal	Neurological Research
Volume	24
Issue number	6
DOIs	https://doi.org/10.1179/016164102101200384
Publication status	Published - Sept 1 2002
Externally published	Yes

Keywords

ALS
EAAT
Glutamate
Glutamate transporter
SOD1
Transgenic mouse

ASJC Scopus subject areas

Neurology
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1179/016164102101200384

Cite this

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title = "Tardive decrease of astrocytic glutamate transporter protein in transgenic mice with ALS-linked mutant SOD1",

abstract = "The expressions of glutamate transporter proteins were immunocytochemically examined in the spinal cord of transgenic mice harboring a Gly93 → Ala (G93A) mutant human SOD1 gene. Astroglial EAAT2 protein level was preserved in the ventral horn even after the beginning of paralysis, and finally decreased at terminal stage of the disease (35 weeks of age), when neuronal EAAT3 protein level was also decreased. In contrast, glial fibrillary acidic protein (GFAP) immunoreactivity progressively increased from 25 weeks of age in the ventral horn. The present results show interesting dissociative expressions of astroglial proteins EAAT2 and GFAP in the same ventral horn, but suggest not an early and primary role of EAAT2 in the motoneuronal death of this model.",

keywords = "ALS, EAAT, Glutamate, Glutamate transporter, SOD1, Transgenic mouse",

author = "H. Warita and Y. Manabe and T. Murakami and M. Shiote and Y. Shiro and T. Hayashi and I. Nagano and M. Shoji and K. Abe",

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T1 - Tardive decrease of astrocytic glutamate transporter protein in transgenic mice with ALS-linked mutant SOD1

AU - Warita, H.

AU - Manabe, Y.

AU - Murakami, T.

AU - Shiote, M.

AU - Shiro, Y.

AU - Hayashi, T.

AU - Nagano, I.

AU - Shoji, M.

AU - Abe, K.

PY - 2002/9/1

Y1 - 2002/9/1

N2 - The expressions of glutamate transporter proteins were immunocytochemically examined in the spinal cord of transgenic mice harboring a Gly93 → Ala (G93A) mutant human SOD1 gene. Astroglial EAAT2 protein level was preserved in the ventral horn even after the beginning of paralysis, and finally decreased at terminal stage of the disease (35 weeks of age), when neuronal EAAT3 protein level was also decreased. In contrast, glial fibrillary acidic protein (GFAP) immunoreactivity progressively increased from 25 weeks of age in the ventral horn. The present results show interesting dissociative expressions of astroglial proteins EAAT2 and GFAP in the same ventral horn, but suggest not an early and primary role of EAAT2 in the motoneuronal death of this model.

AB - The expressions of glutamate transporter proteins were immunocytochemically examined in the spinal cord of transgenic mice harboring a Gly93 → Ala (G93A) mutant human SOD1 gene. Astroglial EAAT2 protein level was preserved in the ventral horn even after the beginning of paralysis, and finally decreased at terminal stage of the disease (35 weeks of age), when neuronal EAAT3 protein level was also decreased. In contrast, glial fibrillary acidic protein (GFAP) immunoreactivity progressively increased from 25 weeks of age in the ventral horn. The present results show interesting dissociative expressions of astroglial proteins EAAT2 and GFAP in the same ventral horn, but suggest not an early and primary role of EAAT2 in the motoneuronal death of this model.

KW - ALS

KW - EAAT

KW - Glutamate

KW - Glutamate transporter

KW - SOD1

KW - Transgenic mouse

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JF - Neurological Research

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Tardive decrease of astrocytic glutamate transporter protein in transgenic mice with ALS-linked mutant SOD1

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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