Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein

Dmitri V. Gordienko; Hirokazu Tsukahara

doi:10.1007/BF00374756

Tetrodotoxin-blockable depolarization-activated Na⁺ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein

Dmitri V. Gordienko, Hirokazu Tsukahara

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Voltage-dependent Na⁺ current (I_Na) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced I_Na in a dose-dependent manner with the apparent dissociation constant (K_d) of 1.4 μM. Low sensitivity of I_Na to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type [2].

Original language	English
Pages (from-to)	91-93
Number of pages	3
Journal	Pflügers Archiv European Journal of Physiology
Volume	428
Issue number	1
DOIs	https://doi.org/10.1007/BF00374756
Publication status	Published - Aug 1 1994
Externally published	Yes

Keywords

Na channel
Patch-clamp technique
Tetrodotoxin
Vascular endothelium

ASJC Scopus subject areas

Physiology
Clinical Biochemistry
Physiology (medical)

Access to Document

10.1007/BF00374756

Cite this

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AB - Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 μM. Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type [2].

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