TY - JOUR
T1 - TGF-β1 and HGF coordinately facilitate collagen turnover in subepithelial mesenchyme
AU - Inoue, Tsutomu
AU - Okada, Hirokazu
AU - Kobayashi, Tatsuya
AU - Watanabe, Yusuke
AU - Kikuta, Tomohiro
AU - Kanno, Yoshihiko
AU - Takigawa, Masaharu
AU - Suzuki, Hiromichi
N1 - Funding Information:
We are grateful to J. Takahashi and M. Otobe for their technical assistance. This work was supported in part by a Grant-in-Aid for Scientific Research from the Japanese Ministry for Education, Science and Culture, and by a Grant from Ochiai Memorial Award 2000 from the Alumni Association of Saitama Medical College.
PY - 2002
Y1 - 2002
N2 - We have employed co-culture of proximal tubular epithelial cells (PTEC) and renal tubulo-interstitial fibroblasts (TFB) to study the role of transforming growth factor-β1 (TGF-β1) and hepatocyte growth factor (HGF) in epithelial-mesenchymal interactions. In co-culture, TGF-β1 stimulated TFB to produce type I collagen (COLI). This effect was both direct and indirect, via connective tissue growth factor (CTGF) produced by the co-cultured PTEC. Co-administration of TGF-β1 and HGF significantly increased overall COLI production by TFB by 24 h. However, in detail, this co-administration enhanced CTGF induction in PTEC during the first 8 h, and then decreased its expression, resulting in a rapid decrease in expression of the α1(I) procollagen gene in TFB by 24 h. Additionally, tissue inhibitor of metalloproteinase-1 was induced in PTEC by TGF-β1 with or without co-administration of HGF, which contributed to the COLI accumulation. In contrast, HGF alone or co-administered with TGF-β1 significantly increased collagenolytic activity derived from PTEC. Therefore, TGF-β1 and HGF seem to coordinately modulate epithelial-mesenchymal interactions to facilitate COLI turnover in subepithelial mesenchyme.
AB - We have employed co-culture of proximal tubular epithelial cells (PTEC) and renal tubulo-interstitial fibroblasts (TFB) to study the role of transforming growth factor-β1 (TGF-β1) and hepatocyte growth factor (HGF) in epithelial-mesenchymal interactions. In co-culture, TGF-β1 stimulated TFB to produce type I collagen (COLI). This effect was both direct and indirect, via connective tissue growth factor (CTGF) produced by the co-cultured PTEC. Co-administration of TGF-β1 and HGF significantly increased overall COLI production by TFB by 24 h. However, in detail, this co-administration enhanced CTGF induction in PTEC during the first 8 h, and then decreased its expression, resulting in a rapid decrease in expression of the α1(I) procollagen gene in TFB by 24 h. Additionally, tissue inhibitor of metalloproteinase-1 was induced in PTEC by TGF-β1 with or without co-administration of HGF, which contributed to the COLI accumulation. In contrast, HGF alone or co-administered with TGF-β1 significantly increased collagenolytic activity derived from PTEC. Therefore, TGF-β1 and HGF seem to coordinately modulate epithelial-mesenchymal interactions to facilitate COLI turnover in subepithelial mesenchyme.
KW - Co-culture
KW - Collagenase
KW - Connective tissue growth factor
KW - Fibroblasts
KW - Hepatocyte growth factor
KW - Tissue inhibitor of metalloproteinase-1
KW - Transforming growth factor-β1
KW - Tubular epithelial cells
KW - Type I collagen
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U2 - 10.1016/S0006-291X(02)02192-7
DO - 10.1016/S0006-291X(02)02192-7
M3 - Article
C2 - 12237111
AN - SCOPUS:0036025333
SN - 0006-291X
VL - 297
SP - 255
EP - 260
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -