TGF-β1 and HGF coordinately facilitate collagen turnover in subepithelial mesenchyme

Tsutomu Inoue, Hirokazu Okada, Tatsuya Kobayashi, Yusuke Watanabe, Tomohiro Kikuta, Yoshihiko Kanno, Masaharu Takigawa, Hiromichi Suzuki

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


We have employed co-culture of proximal tubular epithelial cells (PTEC) and renal tubulo-interstitial fibroblasts (TFB) to study the role of transforming growth factor-β1 (TGF-β1) and hepatocyte growth factor (HGF) in epithelial-mesenchymal interactions. In co-culture, TGF-β1 stimulated TFB to produce type I collagen (COLI). This effect was both direct and indirect, via connective tissue growth factor (CTGF) produced by the co-cultured PTEC. Co-administration of TGF-β1 and HGF significantly increased overall COLI production by TFB by 24 h. However, in detail, this co-administration enhanced CTGF induction in PTEC during the first 8 h, and then decreased its expression, resulting in a rapid decrease in expression of the α1(I) procollagen gene in TFB by 24 h. Additionally, tissue inhibitor of metalloproteinase-1 was induced in PTEC by TGF-β1 with or without co-administration of HGF, which contributed to the COLI accumulation. In contrast, HGF alone or co-administered with TGF-β1 significantly increased collagenolytic activity derived from PTEC. Therefore, TGF-β1 and HGF seem to coordinately modulate epithelial-mesenchymal interactions to facilitate COLI turnover in subepithelial mesenchyme.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 2002


  • Co-culture
  • Collagenase
  • Connective tissue growth factor
  • Fibroblasts
  • Hepatocyte growth factor
  • Tissue inhibitor of metalloproteinase-1
  • Transforming growth factor-β1
  • Tubular epithelial cells
  • Type I collagen

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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