TY - JOUR
T1 - The 1.9 Å X-ray structure of egg-white lysozyme from Taiwanese soft-shelled turtle (Trionyx sinensis wiegmann) exhibits structural differences from the standard chicken-type lysozyme
AU - Siritapetawee, Jaruwan
AU - Thammasirirak, Sompong
AU - Robinson, Robert C.
AU - Yuvaniyama, Jirundon
N1 - Funding Information:
This work was partly supported by Mahidol University, Thailand (to J.Y.); and A*STAR, Singapore (to R.C.R.).
PY - 2009/2
Y1 - 2009/2
N2 - Lysozyme from Taiwanese soft-shelled turtle (SSTLB) has been purified from turtle egg white and crystallized. The crystals diffract X-rays beyond 2 Å resolution and belong to the orthorhombic P21212 1 space group containing one molecule per asymmetric unit. The structure was elucidated using pheasant egg-white lysozyme as the molecular replacement search template. The overall structure of SSTLB is very similar to that of hen egg-white lysozyme (HEWL). Nevertheless, Pro104 in the substrate subsite A and other amino acids in the substrate subsites E and F differ from those of HEWL. These substitutions result in local conformational differences in the substrate binding sites of the two enzymes, effecting substrate binding and transglycosylation efficiency, translating into differing ranges of products.
AB - Lysozyme from Taiwanese soft-shelled turtle (SSTLB) has been purified from turtle egg white and crystallized. The crystals diffract X-rays beyond 2 Å resolution and belong to the orthorhombic P21212 1 space group containing one molecule per asymmetric unit. The structure was elucidated using pheasant egg-white lysozyme as the molecular replacement search template. The overall structure of SSTLB is very similar to that of hen egg-white lysozyme (HEWL). Nevertheless, Pro104 in the substrate subsite A and other amino acids in the substrate subsites E and F differ from those of HEWL. These substitutions result in local conformational differences in the substrate binding sites of the two enzymes, effecting substrate binding and transglycosylation efficiency, translating into differing ranges of products.
KW - Crystal structure
KW - Lysozyme
KW - Soft-shelled turtle
KW - Transglycosylation
KW - X-ray crystallography
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U2 - 10.1093/jb/mvn156
DO - 10.1093/jb/mvn156
M3 - Article
C2 - 19029145
AN - SCOPUS:59449103449
SN - 0021-924X
VL - 145
SP - 193
EP - 198
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 2
ER -