The aim of the measurement of Epstein-Barr virus DNA in hydroa vacciniforme and hypersensitivity to mosquito bites

Tomoko Miyake; Keiji Iwatsuki; Yoji Hirai; Takenobu Yamamoto; Toshihisa Hamada; Kazuyasu Fujii; Hideaki Imamura; Shin Morizane

doi:10.1002/jmv.25811

The aim of the measurement of Epstein-Barr virus DNA in hydroa vacciniforme and hypersensitivity to mosquito bites

Tomoko Miyake, Keiji Iwatsuki, Yoji Hirai, Takenobu Yamamoto, Toshihisa Hamada, Kazuyasu Fujii, Hideaki Imamura, Shin Morizane

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosquito bites (HMB) to understand the clinical significance of EBV DNA load. All 30 patients showed high DNA loads in the PBMCs over the cut-off level. Of 16 plasma samples, extremely high in two samples obtained from patients with hemophagocytic lymphohistiocytosis (HLH). The amount of cell-free DNA in plasma was correlated to the serum levels of lactate dehydrogenase and inversely correlated to platelet counts. These results indicate that the EBV DNA load in PBMCs can provide one of the diagnostic indicators for HV and HMB and marked elevation of cell-free EBV DNA in plasma might be related to cytolysis such as that observed in HLH.

Original language	English
Pages (from-to)	3689-3696
Number of pages	8
Journal	Journal of Medical Virology
Volume	92
Issue number	12
DOIs	https://doi.org/10.1002/jmv.25811
Publication status	Published - Dec 1 2020

Keywords

Epstein-Barr virus DNA load
hemophagocytic lymphohistiocytosis
hydroa vacciniforme
hypersensitivity to mosquito bite

ASJC Scopus subject areas

Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jmv.25811

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title = "The aim of the measurement of Epstein-Barr virus DNA in hydroa vacciniforme and hypersensitivity to mosquito bites",

abstract = "Epstein-Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosquito bites (HMB) to understand the clinical significance of EBV DNA load. All 30 patients showed high DNA loads in the PBMCs over the cut-off level. Of 16 plasma samples, extremely high in two samples obtained from patients with hemophagocytic lymphohistiocytosis (HLH). The amount of cell-free DNA in plasma was correlated to the serum levels of lactate dehydrogenase and inversely correlated to platelet counts. These results indicate that the EBV DNA load in PBMCs can provide one of the diagnostic indicators for HV and HMB and marked elevation of cell-free EBV DNA in plasma might be related to cytolysis such as that observed in HLH.",

keywords = "Epstein-Barr virus DNA load, hemophagocytic lymphohistiocytosis, hydroa vacciniforme, hypersensitivity to mosquito bite",

author = "Tomoko Miyake and Keiji Iwatsuki and Yoji Hirai and Takenobu Yamamoto and Toshihisa Hamada and Kazuyasu Fujii and Hideaki Imamura and Shin Morizane",

note = "Funding Information: The authors would like to thank Dr Mitsuhashi Toshiharu (Okayama University, Center for Innovative Clinical Medicine) for technical advice and Naoko Kawahara, Yoshiko Fujitani, and Hiroko Katayama for technical assistance. This study was supported by AMED (17ek0109098 and 17ek0109124), MHLW (H29‐Measures for Rare and Intractable Diseases‐016), and MEXT (15k09744, 17K16431, and 18K16063), GSK grant (B‐47). Funding Information: The authors would like to thank Dr Mitsuhashi Toshiharu (Okayama University, Center for Innovative Clinical Medicine) for technical advice and Naoko Kawahara, Yoshiko Fujitani, and Hiroko Katayama for technical assistance. This study was supported by AMED (17ek0109098 and 17ek0109124), MHLW (H29-Measures for Rare and Intractable Diseases-016), and MEXT (15k09744, 17K16431, and 18K16063), GSK grant (B-47). Publisher Copyright: {\textcopyright} 2020 Wiley Periodicals, Inc.",

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AU - Miyake, Tomoko

AU - Iwatsuki, Keiji

AU - Hirai, Yoji

AU - Yamamoto, Takenobu

AU - Hamada, Toshihisa

AU - Fujii, Kazuyasu

AU - Imamura, Hideaki

AU - Morizane, Shin

N1 - Funding Information: The authors would like to thank Dr Mitsuhashi Toshiharu (Okayama University, Center for Innovative Clinical Medicine) for technical advice and Naoko Kawahara, Yoshiko Fujitani, and Hiroko Katayama for technical assistance. This study was supported by AMED (17ek0109098 and 17ek0109124), MHLW (H29‐Measures for Rare and Intractable Diseases‐016), and MEXT (15k09744, 17K16431, and 18K16063), GSK grant (B‐47). Funding Information: The authors would like to thank Dr Mitsuhashi Toshiharu (Okayama University, Center for Innovative Clinical Medicine) for technical advice and Naoko Kawahara, Yoshiko Fujitani, and Hiroko Katayama for technical assistance. This study was supported by AMED (17ek0109098 and 17ek0109124), MHLW (H29-Measures for Rare and Intractable Diseases-016), and MEXT (15k09744, 17K16431, and 18K16063), GSK grant (B-47). Publisher Copyright: © 2020 Wiley Periodicals, Inc.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Epstein-Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosquito bites (HMB) to understand the clinical significance of EBV DNA load. All 30 patients showed high DNA loads in the PBMCs over the cut-off level. Of 16 plasma samples, extremely high in two samples obtained from patients with hemophagocytic lymphohistiocytosis (HLH). The amount of cell-free DNA in plasma was correlated to the serum levels of lactate dehydrogenase and inversely correlated to platelet counts. These results indicate that the EBV DNA load in PBMCs can provide one of the diagnostic indicators for HV and HMB and marked elevation of cell-free EBV DNA in plasma might be related to cytolysis such as that observed in HLH.

AB - Epstein-Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosquito bites (HMB) to understand the clinical significance of EBV DNA load. All 30 patients showed high DNA loads in the PBMCs over the cut-off level. Of 16 plasma samples, extremely high in two samples obtained from patients with hemophagocytic lymphohistiocytosis (HLH). The amount of cell-free DNA in plasma was correlated to the serum levels of lactate dehydrogenase and inversely correlated to platelet counts. These results indicate that the EBV DNA load in PBMCs can provide one of the diagnostic indicators for HV and HMB and marked elevation of cell-free EBV DNA in plasma might be related to cytolysis such as that observed in HLH.

KW - Epstein-Barr virus DNA load

KW - hemophagocytic lymphohistiocytosis

KW - hydroa vacciniforme

KW - hypersensitivity to mosquito bite

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The aim of the measurement of Epstein-Barr virus DNA in hydroa vacciniforme and hypersensitivity to mosquito bites

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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