TY - JOUR
T1 - The BF4 and p71 antenna mutants from Chlamydomonas reinhardtii
AU - Bujaldon, Sandrine
AU - Kodama, Natsumi
AU - Rathod, Mithun Kumar
AU - Tourasse, Nicolas
AU - Ozawa, Shin Ichiro
AU - Sellés, Julien
AU - Vallon, Olivier
AU - Takahashi, Yuichiro
AU - Wollman, Francis André
N1 - Funding Information:
This work was supported by the ‘Initiative d'Excellence’ program from the French State (Grant ‘DYNAMO’ , ANR-11-LABX-0011-01 ) and by JSPS KAKENHI Grant Number 16H06554 . We thank Alberta Pinnola for providing the antibodies to LhcSR proteins. Appendix A
Funding Information:
This work was supported by the ?Initiative d'Excellence? program from the French State (Grant ?DYNAMO?, ANR-11-LABX-0011-01) and by JSPS KAKENHI Grant Number 16H06554. We thank Alberta Pinnola for providing the antibodies to LhcSR proteins.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Two pale green mutants of the green alga Chlamydomonas reinhardtii, which have been used over the years in many photosynthesis studies, the BF4 and p71 mutants, were characterized and their mutated gene identified in the nuclear genome. The BF4 mutant is defective in the insertase Alb3.1 whereas p71 is defective in cpSRP43. The two mutants showed strikingly similar deficiencies in most of the peripheral antenna proteins associated with either photosystem I or photosystem 2. As a result the two photosystems have a reduced antenna size with photosystem 2 being the most affected. Still up to 20% of the antenna proteins remain in these strains, with the heterodimer Lhca5/Lhca6 showing a lower sensitivity to these mutations. We discuss these phenotypes in light of those of other allelic mutants that have been described in the literature and suggest that eventhough the cpSRP route serves as the main biogenesis pathway for antenna proteins, there should be an escape pathway which remains to be genetically identified.
AB - Two pale green mutants of the green alga Chlamydomonas reinhardtii, which have been used over the years in many photosynthesis studies, the BF4 and p71 mutants, were characterized and their mutated gene identified in the nuclear genome. The BF4 mutant is defective in the insertase Alb3.1 whereas p71 is defective in cpSRP43. The two mutants showed strikingly similar deficiencies in most of the peripheral antenna proteins associated with either photosystem I or photosystem 2. As a result the two photosystems have a reduced antenna size with photosystem 2 being the most affected. Still up to 20% of the antenna proteins remain in these strains, with the heterodimer Lhca5/Lhca6 showing a lower sensitivity to these mutations. We discuss these phenotypes in light of those of other allelic mutants that have been described in the literature and suggest that eventhough the cpSRP route serves as the main biogenesis pathway for antenna proteins, there should be an escape pathway which remains to be genetically identified.
KW - ALB3
KW - Antenna
KW - Chlamydomonas
KW - Light harvesting complex
KW - Photosynthesis
KW - cpSRP
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U2 - 10.1016/j.bbabio.2019.148085
DO - 10.1016/j.bbabio.2019.148085
M3 - Article
C2 - 31672413
AN - SCOPUS:85075385119
SN - 0005-2728
VL - 1861
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 4
M1 - 148085
ER -