TY - JOUR
T1 - The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process
AU - Ochi, Anna
AU - Makabe, Koki
AU - Yamagami, Ryota
AU - Hirata, Akira
AU - Sakaguchi, Reiko
AU - Hou, Ya Ming
AU - Watanabe, Kazunori
AU - Nureki, Osamu
AU - Kuwajima, Kunihiro
AU - Hori, Hiroyuki
PY - 2013/8/30
Y1 - 2013/8/30
N2 - Background: Topologically knotted tRNA methyltransferases specifically recognize substrate tRNA. Results: Site-directed mutagenesis studies, chimeric protein analysis, and pre-steady state kinetics clarify the tRNA recognition sites of TrmH. Conclusion: The N- and C-terminal regions function in the initial binding process, and substrate tRNA is discriminated by the catalytic domain in an induced-fit process. Significance: Study of how proteins recognize RNA is crucial for understanding RNA maturation processes.
AB - Background: Topologically knotted tRNA methyltransferases specifically recognize substrate tRNA. Results: Site-directed mutagenesis studies, chimeric protein analysis, and pre-steady state kinetics clarify the tRNA recognition sites of TrmH. Conclusion: The N- and C-terminal regions function in the initial binding process, and substrate tRNA is discriminated by the catalytic domain in an induced-fit process. Significance: Study of how proteins recognize RNA is crucial for understanding RNA maturation processes.
UR - http://www.scopus.com/inward/record.url?scp=84883372113&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883372113&partnerID=8YFLogxK
U2 - 10.1074/jbc.M113.485128
DO - 10.1074/jbc.M113.485128
M3 - Article
C2 - 23867454
AN - SCOPUS:84883372113
SN - 0021-9258
VL - 288
SP - 25562
EP - 25574
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 35
ER -