TY - JOUR
T1 - The co-expression of P2X3 receptor with VR1 and VRL-1 in the rat trigeminal ganglion
AU - Ichikawa, Hiroyuki
AU - Sugimoto, Tomosada
N1 - Funding Information:
We greatly appreciate Dr. David Julius at the Department of Cellular and Molecular Pharmacology, University of California, for his kind gift of the anti-VRL-1-serum. This work was supported by a grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology to H.I. (No. 14571733).
PY - 2004/2/13
Y1 - 2004/2/13
N2 - The co-expression of P2X3 receptor with the vanilloid receptor subtype I (VR1) and vanilloid receptor 1-like receptor (VRL-1) was examined in the rat trigeminal ganglion (TG) by a double immunofluorescence method. P2X3 receptor-immunoreactive (ir) neurons were predominantly small to medium-sized (range=93.8-1844.4 μm2, mean±S.D.=503.8±286.5 μm2); 35% and 9% of P2X3 receptor-ir TG neurons were immunoreactive for VR1 and VRL-1, respectively. Small and medium-sized P2X3 receptor-ir neurons contained VR1-immunoreactivity (ir), whereas medium-sized and large P2X3 receptor-ir neurons showed VRL-1-ir. The retrograde tracing and immunohistochemical methods revealed that 30% of the TG neurons retrogradely labeled from the facial skin and tooth pulp exhibited P2X3 receptor-ir. The co-expression of P2X3 receptor and VR1 was detected in 16% of cutaneous TG neurons and 6% of tooth pulp neurons. On the other hand, the co-expression of P2X3 receptor and VRL-1 was common in tooth pulp neurons (23%) and rare in cutaneous TG neurons (8%). In the tooth pulp, 95% of P2X3 receptor-ir TG neurons contained VRL-1-ir. The present study indicates that P2X3 receptor-ir TG neurons, which co-express VR-ir, are abundant in the facial skin. The tooth pulp is probably innervated by TG neurons, which contain both P2X3-and VRL-1-ir.
AB - The co-expression of P2X3 receptor with the vanilloid receptor subtype I (VR1) and vanilloid receptor 1-like receptor (VRL-1) was examined in the rat trigeminal ganglion (TG) by a double immunofluorescence method. P2X3 receptor-immunoreactive (ir) neurons were predominantly small to medium-sized (range=93.8-1844.4 μm2, mean±S.D.=503.8±286.5 μm2); 35% and 9% of P2X3 receptor-ir TG neurons were immunoreactive for VR1 and VRL-1, respectively. Small and medium-sized P2X3 receptor-ir neurons contained VR1-immunoreactivity (ir), whereas medium-sized and large P2X3 receptor-ir neurons showed VRL-1-ir. The retrograde tracing and immunohistochemical methods revealed that 30% of the TG neurons retrogradely labeled from the facial skin and tooth pulp exhibited P2X3 receptor-ir. The co-expression of P2X3 receptor and VR1 was detected in 16% of cutaneous TG neurons and 6% of tooth pulp neurons. On the other hand, the co-expression of P2X3 receptor and VRL-1 was common in tooth pulp neurons (23%) and rare in cutaneous TG neurons (8%). In the tooth pulp, 95% of P2X3 receptor-ir TG neurons contained VRL-1-ir. The present study indicates that P2X3 receptor-ir TG neurons, which co-express VR-ir, are abundant in the facial skin. The tooth pulp is probably innervated by TG neurons, which contain both P2X3-and VRL-1-ir.
KW - Facial skin
KW - Immunohistochemistry
KW - P2X3 receptor
KW - Tooth pulp
KW - Trigeminal ganglion
KW - VR1
KW - VRL-1
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U2 - 10.1016/j.brainres.2003.11.019
DO - 10.1016/j.brainres.2003.11.019
M3 - Article
C2 - 14725976
AN - SCOPUS:0347914629
SN - 0006-8993
VL - 998
SP - 130
EP - 135
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -