The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke

Kazuhiko Nakano; Kazuya Hokamura; Naho Taniguchi; Koichiro Wada; Chiho Kudo; Ryota Nomura; Ayuchi Kojima; Shuhei Naka; Yoshinori Muranaka; Min Thura; Atsushi Nakajima; Katsuhiko Masuda; Ichiro Nakagawa; Pietro Speziale; Nobumitsu Shimada; Atsuo Amano; Yoshinori Kamisaki; Tokutaro Tanaka; Kazuo Umemura; Takashi Ooshima

doi:10.1038/ncomms1491

The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke

Kazuhiko Nakano, Kazuya Hokamura, Naho Taniguchi, Koichiro Wada, Chiho Kudo, Ryota Nomura, Ayuchi Kojima, Shuhei Naka, Yoshinori Muranaka, Min Thura, Atsushi Nakajima, Katsuhiko Masuda, Ichiro Nakagawa, Pietro Speziale, Nobumitsu Shimada, Atsuo Amano, Yoshinori Kamisaki, Tokutaro Tanaka, Kazuo Umemura, Takashi Ooshima

Research output: Contribution to journal › Article › peer-review

121 Citations (Scopus)

Abstract

Although several risk factors for stroke have been identified, one-third remain unexplained. Here we show that infection with Streptococcus mutans expressing collagen-binding protein (CBP) is a potential risk factor for haemorrhagic stroke. Infection with serotype k S. mutans, but not a standard strain, aggravates cerebral haemorrhage in mice. Serotype k S. mutans accumulates in the damaged, but not the contralateral hemisphere, indicating an interaction of bacteria with injured blood vessels. The most important factor for high-virulence is expression of CBP, which is a common property of most serotype k strains. The detection frequency of CBP-expressing S. mutans in haemorrhagic stroke patients is significantly higher than in control subjects. Strains isolated from haemorrhagic stroke patients aggravate haemorrhage in a mouse model, indicating that they are haemorrhagic stroke-associated. Administration of recombinant CBP causes aggravation of haemorrhage. Our data suggest that CBP of S. mutans is directly involved in haemorrhagic stroke.

Original language	English
Article number	485
Journal	Nature communications
Volume	2
Issue number	1
DOIs	https://doi.org/10.1038/ncomms1491
Publication status	Published - 2011
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/ncomms1491

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Nakano, K., Hokamura, K., Taniguchi, N., Wada, K., Kudo, C., Nomura, R., Kojima, A., Naka, S., Muranaka, Y., Thura, M., Nakajima, A., Masuda, K., Nakagawa, I., Speziale, P., Shimada, N., Amano, A., Kamisaki, Y., Tanaka, T., Umemura, K., & Ooshima, T. (2011). The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke. Nature communications, 2(1), Article 485. https://doi.org/10.1038/ncomms1491

Nakano, K, Hokamura, K, Taniguchi, N, Wada, K, Kudo, C, Nomura, R, Kojima, A, Naka, S, Muranaka, Y, Thura, M, Nakajima, A, Masuda, K, Nakagawa, I, Speziale, P, Shimada, N, Amano, A, Kamisaki, Y, Tanaka, T, Umemura, K & Ooshima, T 2011, 'The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke', Nature communications, vol. 2, no. 1, 485. https://doi.org/10.1038/ncomms1491

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title = "The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke",

abstract = "Although several risk factors for stroke have been identified, one-third remain unexplained. Here we show that infection with Streptococcus mutans expressing collagen-binding protein (CBP) is a potential risk factor for haemorrhagic stroke. Infection with serotype k S. mutans, but not a standard strain, aggravates cerebral haemorrhage in mice. Serotype k S. mutans accumulates in the damaged, but not the contralateral hemisphere, indicating an interaction of bacteria with injured blood vessels. The most important factor for high-virulence is expression of CBP, which is a common property of most serotype k strains. The detection frequency of CBP-expressing S. mutans in haemorrhagic stroke patients is significantly higher than in control subjects. Strains isolated from haemorrhagic stroke patients aggravate haemorrhage in a mouse model, indicating that they are haemorrhagic stroke-associated. Administration of recombinant CBP causes aggravation of haemorrhage. Our data suggest that CBP of S. mutans is directly involved in haemorrhagic stroke.",

author = "Kazuhiko Nakano and Kazuya Hokamura and Naho Taniguchi and Koichiro Wada and Chiho Kudo and Ryota Nomura and Ayuchi Kojima and Shuhei Naka and Yoshinori Muranaka and Min Thura and Atsushi Nakajima and Katsuhiko Masuda and Ichiro Nakagawa and Pietro Speziale and Nobumitsu Shimada and Atsuo Amano and Yoshinori Kamisaki and Tokutaro Tanaka and Kazuo Umemura and Takashi Ooshima",

note = "Funding Information: We thank Dr Michael J. Moskowitz at Harvard Medical School and Dr Tatsuhiko Kodama at Tokyo University for their helpful suggestions and discussions. This study was supported by Grants-in-Aid for Scientific Research (A) 19209063, (C) 21592357, (C) 20591683 from the Japan Society for Promotion of Science, and Grant-in-Aid for Young Scientists (A) 21689052 and (B) 21792067 from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We are grateful to Dr Shoichi Nakamura, Otsuka Electronics, for his technical support and assistance in evaluating the zeta-potential of the bacterial cells.",

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AU - Nakano, Kazuhiko

AU - Hokamura, Kazuya

AU - Taniguchi, Naho

AU - Wada, Koichiro

AU - Kudo, Chiho

AU - Nomura, Ryota

AU - Kojima, Ayuchi

AU - Naka, Shuhei

AU - Muranaka, Yoshinori

AU - Thura, Min

AU - Nakajima, Atsushi

AU - Masuda, Katsuhiko

AU - Nakagawa, Ichiro

AU - Speziale, Pietro

AU - Shimada, Nobumitsu

AU - Amano, Atsuo

AU - Kamisaki, Yoshinori

AU - Tanaka, Tokutaro

AU - Umemura, Kazuo

AU - Ooshima, Takashi

N1 - Funding Information: We thank Dr Michael J. Moskowitz at Harvard Medical School and Dr Tatsuhiko Kodama at Tokyo University for their helpful suggestions and discussions. This study was supported by Grants-in-Aid for Scientific Research (A) 19209063, (C) 21592357, (C) 20591683 from the Japan Society for Promotion of Science, and Grant-in-Aid for Young Scientists (A) 21689052 and (B) 21792067 from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We are grateful to Dr Shoichi Nakamura, Otsuka Electronics, for his technical support and assistance in evaluating the zeta-potential of the bacterial cells.

PY - 2011

Y1 - 2011

N2 - Although several risk factors for stroke have been identified, one-third remain unexplained. Here we show that infection with Streptococcus mutans expressing collagen-binding protein (CBP) is a potential risk factor for haemorrhagic stroke. Infection with serotype k S. mutans, but not a standard strain, aggravates cerebral haemorrhage in mice. Serotype k S. mutans accumulates in the damaged, but not the contralateral hemisphere, indicating an interaction of bacteria with injured blood vessels. The most important factor for high-virulence is expression of CBP, which is a common property of most serotype k strains. The detection frequency of CBP-expressing S. mutans in haemorrhagic stroke patients is significantly higher than in control subjects. Strains isolated from haemorrhagic stroke patients aggravate haemorrhage in a mouse model, indicating that they are haemorrhagic stroke-associated. Administration of recombinant CBP causes aggravation of haemorrhage. Our data suggest that CBP of S. mutans is directly involved in haemorrhagic stroke.

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The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke

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