The deep red state of photosystem II in Cyanidioschyzon merolae

J. Langley; J. Morton; R. Purchase; L. Tian; L. Shen; G. Han; J. R. Shen; E. Krausz

doi:10.1007/s11099-017-0760-x

The deep red state of photosystem II in Cyanidioschyzon merolae

J. Langley, J. Morton, R. Purchase, L. Tian, L. Shen, G. Han, J. R. Shen, E. Krausz

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We identified and characterised the deep red state (DRS), an optically-absorbing charge transfer state of PSII, which lies at lower energy than P680, in the red algae Cyanidioschyzon merolae by means of low temperature absorption and magnetic circular dichroism spectroscopies. The photoactive DRS has been previously studied in PSII of the higher plant Spinacia oleracea, and in the cyanobacterium Thermosynechococcus vulcanus. We found the DRS in PSII of C. merolae has similar spectral properties. Treatment of PSII with dithionite leads to reduction of cytochrome (cyt) b₅₅₉ and the PsbV-based cyt c₅₅₀ as well as the disassembly of the oxygen-evolving complex. Whereas the overall visible absorption spectrum of PSII was little affected, the DRS absorption in the reduced sample was no longer seen. This bleaching of the DRS is discussed in terms of a corresponding lack of a DRS feature in D1D2/cyt b₅₅₉ reaction centre preparations of PSII.

Original language	English
Pages (from-to)	275-278
Number of pages	4
Journal	Photosynthetica
Volume	56
Issue number	1
DOIs	https://doi.org/10.1007/s11099-017-0760-x
Publication status	Published - Mar 1 2018

Keywords

optical spectra
photosynthesis

ASJC Scopus subject areas

Physiology
Plant Science

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title = "The deep red state of photosystem II in Cyanidioschyzon merolae",

abstract = "We identified and characterised the deep red state (DRS), an optically-absorbing charge transfer state of PSII, which lies at lower energy than P680, in the red algae Cyanidioschyzon merolae by means of low temperature absorption and magnetic circular dichroism spectroscopies. The photoactive DRS has been previously studied in PSII of the higher plant Spinacia oleracea, and in the cyanobacterium Thermosynechococcus vulcanus. We found the DRS in PSII of C. merolae has similar spectral properties. Treatment of PSII with dithionite leads to reduction of cytochrome (cyt) b559 and the PsbV-based cyt c550 as well as the disassembly of the oxygen-evolving complex. Whereas the overall visible absorption spectrum of PSII was little affected, the DRS absorption in the reduced sample was no longer seen. This bleaching of the DRS is discussed in terms of a corresponding lack of a DRS feature in D1D2/cyt b559 reaction centre preparations of PSII.",

keywords = "optical spectra, photosynthesis",

author = "J. Langley and J. Morton and R. Purchase and L. Tian and L. Shen and G. Han and Shen, {J. R.} and E. Krausz",

note = "Funding Information: Abbreviations: Chl – chlorophyll; cyt – cytochrome; DRS – deep red state; MCD – magnetic circular dichroism; OD – optical density; OEC – oxygen-evolving complex; RC – reaction centre. Acknowledgements: E. Krausz would like to thank Govindjee for bringing the work eventually published in reference (Pettai et al. 2005) to his attention whilst both attended the Photosynthesis Congress in Montreal in 2004. We recognise the support of the Australian Research Council through grants DP110104565 and DP 150103137 (E. Krausz), a Strategic Priority Research Program of CAS (XDB17030100) and a CAS Key Research Project for Frontier Science (QYZDY-SSW-SMC003) of China, and MEXT/JSPS of Japan through a Grant-in-Aid for Specially Promoted Research No. 24000018 (J.-R.S.). Publisher Copyright: {\textcopyright} 2018, The Institute of Experimental Botany.",

year = "2018",

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doi = "10.1007/s11099-017-0760-x",

language = "English",

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AU - Langley, J.

AU - Morton, J.

AU - Purchase, R.

AU - Tian, L.

AU - Shen, L.

AU - Han, G.

AU - Shen, J. R.

AU - Krausz, E.

N1 - Funding Information: Abbreviations: Chl – chlorophyll; cyt – cytochrome; DRS – deep red state; MCD – magnetic circular dichroism; OD – optical density; OEC – oxygen-evolving complex; RC – reaction centre. Acknowledgements: E. Krausz would like to thank Govindjee for bringing the work eventually published in reference (Pettai et al. 2005) to his attention whilst both attended the Photosynthesis Congress in Montreal in 2004. We recognise the support of the Australian Research Council through grants DP110104565 and DP 150103137 (E. Krausz), a Strategic Priority Research Program of CAS (XDB17030100) and a CAS Key Research Project for Frontier Science (QYZDY-SSW-SMC003) of China, and MEXT/JSPS of Japan through a Grant-in-Aid for Specially Promoted Research No. 24000018 (J.-R.S.). Publisher Copyright: © 2018, The Institute of Experimental Botany.

PY - 2018/3/1

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N2 - We identified and characterised the deep red state (DRS), an optically-absorbing charge transfer state of PSII, which lies at lower energy than P680, in the red algae Cyanidioschyzon merolae by means of low temperature absorption and magnetic circular dichroism spectroscopies. The photoactive DRS has been previously studied in PSII of the higher plant Spinacia oleracea, and in the cyanobacterium Thermosynechococcus vulcanus. We found the DRS in PSII of C. merolae has similar spectral properties. Treatment of PSII with dithionite leads to reduction of cytochrome (cyt) b559 and the PsbV-based cyt c550 as well as the disassembly of the oxygen-evolving complex. Whereas the overall visible absorption spectrum of PSII was little affected, the DRS absorption in the reduced sample was no longer seen. This bleaching of the DRS is discussed in terms of a corresponding lack of a DRS feature in D1D2/cyt b559 reaction centre preparations of PSII.

AB - We identified and characterised the deep red state (DRS), an optically-absorbing charge transfer state of PSII, which lies at lower energy than P680, in the red algae Cyanidioschyzon merolae by means of low temperature absorption and magnetic circular dichroism spectroscopies. The photoactive DRS has been previously studied in PSII of the higher plant Spinacia oleracea, and in the cyanobacterium Thermosynechococcus vulcanus. We found the DRS in PSII of C. merolae has similar spectral properties. Treatment of PSII with dithionite leads to reduction of cytochrome (cyt) b559 and the PsbV-based cyt c550 as well as the disassembly of the oxygen-evolving complex. Whereas the overall visible absorption spectrum of PSII was little affected, the DRS absorption in the reduced sample was no longer seen. This bleaching of the DRS is discussed in terms of a corresponding lack of a DRS feature in D1D2/cyt b559 reaction centre preparations of PSII.

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ER -

The deep red state of photosystem II in Cyanidioschyzon merolae

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