TY - GEN
T1 - The effect of Si(IV) species derived from chitosan-silicate hydrogels on osteoblast behavior
AU - Shirosaki, Yuki
AU - Hayakawa, Satoshi
AU - Osaka, Akiyoshi
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Chitosan-GPTMS (γ-Glycidoxypropyltrimethoxysilane) hybrid hydrogels were synthesized with β-glycerophosphate (β-GP) as the additive agent. Chitosan-GPTMS sols were fluid at room temperature and transformed to hydrogel at 36.5°C in several min. The gelation time of the hydrogels was shortened by the addition of GPTMS. From NMR experiments, this gelation behavior depended on some factors, namely, electrostatic interaction between the phosphate groups of β-GP and the amino groups of chitosan chains, crosslinking between the epoxy groups of GPTMS and the amino groups of chitosan, and polycondensation of the methoxy groups of GPTMS. The Si(IV) released from the hydrogels promoted the cell adhesion and ALP activity of osteoblastic cells MG63.
AB - Chitosan-GPTMS (γ-Glycidoxypropyltrimethoxysilane) hybrid hydrogels were synthesized with β-glycerophosphate (β-GP) as the additive agent. Chitosan-GPTMS sols were fluid at room temperature and transformed to hydrogel at 36.5°C in several min. The gelation time of the hydrogels was shortened by the addition of GPTMS. From NMR experiments, this gelation behavior depended on some factors, namely, electrostatic interaction between the phosphate groups of β-GP and the amino groups of chitosan chains, crosslinking between the epoxy groups of GPTMS and the amino groups of chitosan, and polycondensation of the methoxy groups of GPTMS. The Si(IV) released from the hydrogels promoted the cell adhesion and ALP activity of osteoblastic cells MG63.
KW - Chitosan
KW - Hydrogel
KW - Organic-inorganic hybrid
KW - Osteoblast
KW - Si(IV) species
UR - http://www.scopus.com/inward/record.url?scp=81555221117&partnerID=8YFLogxK
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U2 - 10.4028/www.scientific.net/KEM.493-494.698
DO - 10.4028/www.scientific.net/KEM.493-494.698
M3 - Conference contribution
AN - SCOPUS:81555221117
SN - 9783037852552
T3 - Key Engineering Materials
SP - 698
EP - 702
BT - Bioceramics 23
PB - Trans Tech Publications Ltd
T2 - 23rd Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2011
Y2 - 6 November 2011 through 9 November 2011
ER -