The effects of methylglyoxal on glutathione s-transferase from nicotiana tabacum

Md Anamul Hoque; Misugi Uraji; Mst Nasrin Akhter Banu; Izumi C. Mori; Yoshimasa Nakamura; Yoshiyuki Murata

doi:10.1271/bbb.100393

The effects of methylglyoxal on glutathione s-transferase from nicotiana tabacum

Md Anamul Hoque, Misugi Uraji, Mst Nasrin Akhter Banu, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Methylglyoxal (MG) is one of the aldehydes that accumulate in plants under environmental stress. Glutathione S-transferases (GSTs) play important roles, including detoxification, in the stress tolerance systems of plants. To determine the effects of MG, we characterized recombinant GST. MG decreased GST activity and thiol contents with increasing K_m. GST can serve as a target of MG modification, which is suppressed by application of reduced glutathione.

Original language	English
Pages (from-to)	2124-2126
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	74
Issue number	10
DOIs	https://doi.org/10.1271/bbb.100393
Publication status	Published - 2010

Keywords

1-chloro-2,4-dinitrobenzene
Glutathione
Glutathione s-transferase
Methylglyoxal
Thiol

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.100393

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title = "The effects of methylglyoxal on glutathione s-transferase from nicotiana tabacum",

abstract = "Methylglyoxal (MG) is one of the aldehydes that accumulate in plants under environmental stress. Glutathione S-transferases (GSTs) play important roles, including detoxification, in the stress tolerance systems of plants. To determine the effects of MG, we characterized recombinant GST. MG decreased GST activity and thiol contents with increasing Km. GST can serve as a target of MG modification, which is suppressed by application of reduced glutathione.",

keywords = "1-chloro-2,4-dinitrobenzene, Glutathione, Glutathione s-transferase, Methylglyoxal, Thiol",

author = "Hoque, {Md Anamul} and Misugi Uraji and {Akhter Banu}, {Mst Nasrin} and Mori, {Izumi C.} and Yoshimasa Nakamura and Yoshiyuki Murata",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (to YM) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by Research Grants in Postdoctoral Fellowships (to MAH) from the Japan Society for the Promotion of Science.",

year = "2010",

doi = "10.1271/bbb.100393",

language = "English",

volume = "74",

pages = "2124--2126",

journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - The effects of methylglyoxal on glutathione s-transferase from nicotiana tabacum

AU - Hoque, Md Anamul

AU - Uraji, Misugi

AU - Akhter Banu, Mst Nasrin

AU - Mori, Izumi C.

AU - Nakamura, Yoshimasa

AU - Murata, Yoshiyuki

N1 - Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (to YM) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by Research Grants in Postdoctoral Fellowships (to MAH) from the Japan Society for the Promotion of Science.

PY - 2010

Y1 - 2010

N2 - Methylglyoxal (MG) is one of the aldehydes that accumulate in plants under environmental stress. Glutathione S-transferases (GSTs) play important roles, including detoxification, in the stress tolerance systems of plants. To determine the effects of MG, we characterized recombinant GST. MG decreased GST activity and thiol contents with increasing Km. GST can serve as a target of MG modification, which is suppressed by application of reduced glutathione.

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KW - 1-chloro-2,4-dinitrobenzene

KW - Glutathione

KW - Glutathione s-transferase

KW - Methylglyoxal

KW - Thiol

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JO - Bioscience, Biotechnology and Biochemistry

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The effects of methylglyoxal on glutathione s-transferase from nicotiana tabacum

Abstract

Keywords

ASJC Scopus subject areas

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