The excitement of multiple noradrenergic cell groups in the rat brain related to hyperbaric oxygen seizure

Minako Arai, Ken Takata, Yoshimasa Takeda, Satoshi Mizobuchi, Kiyoshi Morita

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The mechanism of oxygen toxicity for central nervous system and hyperbaric oxygen (HBO) seizure has not been clarified. Noradrenergic cells in the brain may contribute to HBO seizure. In this study, we defined the activation of noradrenergic cells during HBO exposure by c-fos immunohistochemistry. Electroencephalogram electrodes were pre-implanted in all animals under general anesthesia. In HBO seizure animals, HBO was induced with 5 atm of 100% oxygen until manifestation of general tonic convulsion. HBO non-seizure animals were exposed to 25 min of HBO. Control animals were put in the chamber for 120 min without pressurization. All animals were processed for c-fos immunohistochemical staining. All animals in the HBO seizure group showed electrical discharge on EEG. In the immunohistochemistry, c-fos was increased in the Al, A2 and A6 cells of the HBO seizure group, and in the A2 and A6 cells of the HBO non-seizure group, yet was extremely low in all three cell types in the control group. These results suggest the participation of noradrenaline in HBO seizure, which can be explained by the early excitement of Al cells due to their higher sensitivity to high blood pressure, hyperoxia, or by the post-seizure activation of all noradrenergic cells.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalActa medica Okayama
Issue number3
Publication statusPublished - Jun 2011


  • Hyperbaric oxygen
  • Immunohistochemistry
  • Noradrenergic cells
  • Seizure

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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