The expression of dentin sialophosphoprotein gene in bone

C. Qin, J. C. Brunn, E. Cadena, A. Ridall, H. Tsujigiwa, H. Nagatsuka, N. Nagai, W. T. Butler

Research output: Contribution to journalArticlepeer-review

106 Citations (Scopus)


Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be tooth-specific. To test for the expression of the dspp gene in bone, we performed Western immunoblots and reverse-transcription polymerase chain-reaction (RT-PCR). With Western immunoblots, we detected DSP in the Gdm/EDTA extracts of rat long bone, at a level of about 1/400 of that in dentin. Using RT-PCR, we detected DSPP mRNA in mouse calvaria. Similar to Western immunoblots, the results of RT-PCR indicated that the dspp gene is expressed at a lower level in bone than in dentin and odontoblasts. Analysis of the data shows that DSPP is not a tooth-specific protein, and that dramatically different regulatory mechanisms governing DSPP expression are involved in the bone and dentin.

Original languageEnglish
Pages (from-to)392-394
Number of pages3
JournalJournal of dental research
Issue number6
Publication statusPublished - 2002
Externally publishedYes


  • Bone
  • Dentin phosphoprotein
  • Dentin sialophosphoprotein
  • Dentin sialoprotein
  • Gene expression

ASJC Scopus subject areas

  • Dentistry(all)


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